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Biotin-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

IF/ICC, ELISA

Conjugate

Biotin

CloneNo.

8H6A10

Cat no : Biotin-66008-4

Print datasheet

Synonyms

验证数据展示

  • ELISA experiment of FusionProtein using Biotin-66008-4

    ELISA experiment of FusionProtein using Biotin-66008-4

    Ag2329 (DYKDDDDK tagged protein) was coated onto microtiter plates at 0.073 µg/well and then incubated with a dilution series of Biotin-66008-4 (start concentration 500 ng/ml). And then detected with Streptavidin Poly-HRP (1:2000) followed by incubation with HRP Substrate, terminated with 2M H2SO4, then measuring the resulting absorbance at 450 nm. Negative control is 66008-4-Ig.

  • IF Staining of Transfected HEK-293 using Biotin-66008-4

    IF Staining of Transfected HEK-293 using Biotin-66008-4

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using Biotin DYKDDDDK tag antibody (Biotin-66008-4, Clone: 8H6A10 ) at dilution of 1:600 and Alexa Fluor 488 Streptavidin.

经过测试的应用

Positive IF/ICC detected inTransfected HEK-293 cells
Positive ELISA detected inFusionProtein

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:2000-1:4000000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-66008-4 targets DYKDDDDK tag in IF/ICC, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity recombinant protein
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name DYKDDDDK tag
Synonyms
Gene Symbol
Gene ID (NCBI)
Conjugate Biotin
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

实验方案

Product Specific Protocols
IF protocol for Biotin DYKDDDDK tag antibody Biotin-66008-4Download protocol
Standard Protocols
Click here to view our Standard Protocols