DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)
DYKDDDDK tag Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
recombinant protein and More (10)
Applications
WB, IF/ICC, FC (Intra), IP, ELISA and More (5)
Conjugate
Unconjugated
293
CloneNo.
8H6A10
验证数据展示
经过测试的应用
| Positive WB detected in | Transfected HEK-293 cells |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected Flag-TDP43 HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66008-4-Ig targets DYKDDDDK tag in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Applications | WB, IF/ICC, FC (Intra), IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP, ChIP, RIP |
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, rat, pig, canine, monkey, chicken, zebrafish, hamster, duck |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | DYKDDDDK tag |
| Synonyms | Flag tag, Flag, DYKDDDDK, DDDDK tag, DDDDK |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2918475 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DYKDDDDK tag antibody 66008-4-Ig | Download protocol |
| IF protocol for DYKDDDDK tag antibody 66008-4-Ig | Download protocol |
| IP protocol for DYKDDDDK tag antibody 66008-4-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Blood Functional, structural and molecular characterizations of leukemogenic driver MEF2D-HNRNPUL1 fusion. | ||
Nat Commun UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean | ||
Nat Commun Homodimer-mediated phosphorylation of C/EBPα-p42 S16 modulates acute myeloid leukaemia differentiation through liquid-liquid phase separation | ||
Adv Sci (Weinh) Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy | ||
Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration | ||
Cell Death Differ Deubiquitinase OTUD6A in macrophages promotes intestinal inflammation and colitis via deubiquitination of NLRP3 |
