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DYKDDDDK tag Monoclonal antibody, PBS Only (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

8H6A10

Cat no : 66008-4-PBS

Print datasheet

Synonyms

Flag, DYKDDDDK, DDDDK tag, DDDDK

验证数据展示

  • WB analysis using 66008-4-Ig (same clone as 66008-4-PBS)

    WB analysis using 66008-4-Ig (same clone as 66008-4-PBS)

    HEK-293 and different loading amount of DYKDDDDK tagged TDP-43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

  • IF Staining of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

    IF Staining of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed DYKDDDDK-TDP43 Transfected HEK-293 cells using DYKDDDDK tag antibody (66008-4-Ig, Clone: 8H6A10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

  • IP experiment of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

    IP experiment of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

    IP result of anti-DYKDDDDK tag (IP:66008-4-Ig, 5 ug; Detection:80010-1-RR 1:10000) with Transfected HEK-293T cells lysate 250 ug. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

  • FC experiment of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

    FC experiment of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

    1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.25 ug DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (66008-4-Ig, Clone:8H6A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

产品信息

66008-4-PBS targets DYKDDDDK tag in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications WB, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity recombinant protein
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name DYKDDDDK tag
Synonyms Flag, DYKDDDDK, DDDDK tag, DDDDK
Gene Symbol
Gene ID (NCBI)
RRIDAB_2918475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.