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验证数据展示

  • IF Staining of Transfected HEK-293 using CL647-66008

    IF Staining of Transfected HEK-293 using CL647-66008

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647 DYKDDDDK tag antibody (CL647-66008, Clone: 8H6A10 ) at dilution of 1:200.

  • FC experiment of Transfected HEK-293T using CL647-66008

    FC experiment of Transfected HEK-293T using CL647-66008

    1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (CL647-66008, Clone:8H6A10). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-66008

产品说明书

CloneNo. 8H6A10
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, FC (Intra) and More (1)

别名

Flag, DYKDDDDK, DDDDK tag, DDDDK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inTransfected HEK-293 cells
Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

CL647-66008 targets DYKDDDDK tag in WB, IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
文献引用应用WB
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Flag Tag
别名 Flag, DYKDDDDK, DDDDK tag, DDDDK
基因名称 Flag Tag
Gene ID (NCBI) 8
RRIDAB_2920272
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein G purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 DYKDDDDK tag antibody CL647-66008Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Adv Sci (Weinh)

FUS Selectively Facilitates circRNAs Packing into Small Extracellular Vesicles within Hypoxia Neuron

Authors - Jiankun Zang
View Article