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CoraLite® Plus 488-conjugated HA tag Polyclonal antibody

HA tag Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-51064

Print datasheet

Synonyms

验证数据展示

  • IF Staining of Transfected HEK-293 using CL488-51064

    IF Staining of Transfected HEK-293 using CL488-51064

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 488 HA tag antibody (CL488-51064) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inTransfected HEK-293 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-51064 targets HA tag in IF/ICC applications and shows reactivity with recombinant protein samples.

Tested Applications IF/ICC Application Description
Tested Reactivity recombinant protein
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HA tag
Synonyms
Calculated Molecular Weight 1 kDa
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HA tag antibody CL488-51064Download protocol
Standard Protocols
Click here to view our Standard Protocols