HA tag Polyclonal antibody
HA tag Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein and More (8)
Applications
IF, IP, WB, ELISA and More (4)
Conjugate
Unconjugated
659
验证数据展示
经过测试的应用
Positive WB detected in | recombinant protein, Transfected HEK-293 cells |
Positive IP detected in | Transfected HEK-293 cells |
Positive IF detected in | Transfected HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
51064-2-AP targets HA tag in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
Tested Applications | IF, IP, WB, ELISA |
Cited Applications | ChIP, CoIP, FC, IF, IHC, IP, WB |
Tested Reactivity | recombinant protein |
Cited Reactivity | human, mouse, rat, chicken, monkey, zebrafish, duck, canine |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | HA tag |
Synonyms | HA tag, YPYDVPDYA |
Calculated Molecular Weight | 1 kDa |
Gene Symbol | |
Gene ID (NCBI) | |
RRID | AB_11042321 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HA tag antibody 51064-2-AP | Download protocol |
IF protocol for HA tag antibody 51064-2-AP | Download protocol |
IP protocol for HA tag antibody 51064-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther FBXW7β loss-of-function enhances FASN-mediated lipogenesis and promotes colorectal cancer growth | ||
Gastroenterology PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis | ||
Nat Genet Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome. |