HA tag Polyclonal antibody

HA tag Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (8)

Applications

IF, IP, WB, ELISA and More (4)

Conjugate

Unconjugated

Cat No : 51064-2-AP

Print datasheet

Synonyms

HA tag, YPYDVPDYA



经过测试的应用

Positive WB detected inrecombinant protein, Transfected HEK-293 cells
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51064-2-AP targets HA tag in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications IF, IP, WB, ELISA
Cited Applications ChIP, CoIP, FC, IF, IHC, IP, WB
Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, rat, chicken, monkey, zebrafish, duck, canine
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HA tag
Synonyms HA tag, YPYDVPDYA
Calculated Molecular Weight 1 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_11042321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

实验方案

Product Specific Protocols
WB protocol for HA tag antibody 51064-2-APDownload protocol
IF protocol for HA tag antibody 51064-2-APDownload protocol
IP protocol for HA tag antibody 51064-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

FBXW7β loss-of-function enhances FASN-mediated lipogenesis and promotes colorectal cancer growth

Authors - Wenxia Wei
WB

Cell

RetroCHMP3 blocks budding of enveloped viruses without blocking cytokinesis.

Authors - Lara Rheinemann
WB

Cell

Glycogen accumulation and phase separation drives liver tumor initiation.

Authors - Qingxu Liu
humanWB

Gastroenterology

PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Authors - Tian-Yi Jiang
WB

Cell

The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.

Authors - Jakob Trendel
WB

Nat Genet

Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome.

Authors - Margot A Cousin