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CoraLite®488-conjugated Alpha Tubulin Monoclonal antibody

Alpha Tubulin Monoclonal Antibody for FC (Intra), IF, WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, Canine

Applications

FC (Intra), IF, WB

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1E4C11

Cat No : CL488-66031

Print datasheet

Synonyms

Alpha Tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, K ALPHA 1, TUBA1B, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b, α-tubulin

验证数据展示

  • IF Staining of mouse embryo using CL488-66031

    IF Staining of mouse embryo using CL488-66031

    E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram

  • IF Staining of HeLa using CL488-66031

    IF Staining of HeLa using CL488-66031

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite®488-conjugated version of this antibody, CL488-66031 (alpha Tubulin antibody), at dilution of 1:100.

  • WB analysis using CL488-66031

    WB analysis using CL488-66031

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using CL488-66031

    FC experiment of HepG2 using CL488-66031

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human Alpha Tubulin (CL488-66031, Clone:1E4C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF detected inHeLa cells, mouse embryo tissue
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66031-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

发表文章中的应用

IFSee 1 publications below

产品信息

The immunogen of CL488-66031 is Alpha Tubulin Fusion Protein expressed in E. coli.

Tested Applications FC (Intra), IF, WB
Cited Applications IF
Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha Tubulin fusion protein Ag18034
Full Name tubulin, alpha 1b
Calculated molecular weight 50 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC009314
Gene symbol TUBA1B
UniprotP68363
Gene ID (NCBI) 10376
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS buffer with 50% glycerol, pH7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.

实验方案

Product Specific Protocols
WB protocol for CL488 Alpha Tubulin antibody CL488-66031Download protocol
IF protocol for CL488 Alpha Tubulin antibody CL488-66031Download protocol
FC protocol for CL488 Alpha Tubulin antibody CL488-66031Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Transl Androl Urol

Novel bi-allelic mutations in DNAH1 cause multiple morphological abnormalities of the sperm flagella resulting in male infertility.

Authors - Chuan Jiang
View Article

Clin Genet

A novel homozygous CFAP65 mutation in humans causes male infertility with multiple morphological abnormalities of the sperm flagella.

Authors - Xueguang Zhang
View Article