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CoraLite®488-conjugated Alpha Tubulin Monoclonal antibody 2 Publications

Mouse Monoclonal| Catalog number: CL488-66031 |CloneNo.: 1E4C11

浓度: 2000 μg/ml

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Applications

Tested Applications:
FC (Intra), IF, WB
Application not tested? Please see our Guarantee


Cited Applications:
IF


Images See all

IF Staining of mouse embryo using CL488-66031

E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram

IF Staining of HeLa using CL488-66031

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite®488-conjugated version of this antibody, CL488-66031 (alpha Tubulin antibody), at dilution of 1:100.

WB analysis using CL488-66031

Various lysates were subjected to SDS PAGE followed by western blot with CL488-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

FC experiment of HepG2 using CL488-66031

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human Alpha Tubulin (CL488-66031, Clone:1E4C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Species Specificity:
human, mouse, rat, Canine
Cited Species:
human
Positive WB detected in:
HepG2 cells, HeLa cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF detected in:
HeLa cells, mouse embryo tissue
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:300-1:600
IF : 1:50-1:500
Excitation/Emission maxima wavelengths:
488 nm/515 nm

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS buffer with 50% glycerol, pH7.3.
Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Alpha Tubulin fusion protein Ag18034
Full name:
tubulin, alpha 1b
Calculated molecular weight:
50 kDa
Observed molecular weight:
50-55 kDa
GenBank accession number:
BC009314
Gene ID (NCBI):
10376
Gene symbol
TUBA1B
Synonyms
Alpha Tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, K ALPHA 1, TUBA1B, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b, α-tubulin

Background

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.

Protocols

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