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CoraLite® Plus 488-conjugated Alpha Tubulin Monoclonal antibody
Alpha Tubulin Monoclonal Antibody for WB, IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, canine
Applications
WB, IF/ICC, FC (Intra) and More (1)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E4C11
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IF/ICC detected in | HeLa cells, mouse embryo tissue |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy TUBA1B Ready-To-Use IHC Kit. TUBA1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66031-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 4 publications below |
产品信息
CL488-66031 targets Alpha Tubulin in WB, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Applications | WB, IF/ICC, FC (Intra) Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat, canine |
| Cited Reactivity | human, mouse |
| Immunogen | Alpha Tubulin fusion protein Ag18034 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | tubulin, alpha 1b |
| Synonyms | TUBA1B, Tubulin alpha 1B chain, Tubulin alpha, EC:3.6.5.-, Detyrosinated tubulin alpha-1B chain |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC009314 |
| Gene Symbol | Alpha Tubulin |
| Gene ID (NCBI) | 10376 |
| RRID | AB_2883232 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P68363 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 Alpha Tubulin antibody CL488-66031 | Download protocol |
| IF protocol for CL Plus 488 Alpha Tubulin antibody CL488-66031 | Download protocol |
| FC protocol for CL Plus 488 Alpha Tubulin antibody CL488-66031 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling | ||
Clin Genet A novel homozygous CFAP65 mutation in humans causes male infertility with multiple morphological abnormalities of the sperm flagella. | ||
Transl Androl Urol Novel bi-allelic mutations in DNAH1 cause multiple morphological abnormalities of the sperm flagella resulting in male infertility. | ||
Reprod Toxicol Bisphenol M inhibits mouse oocyte maturation in vitro by disrupting cytoskeleton architecture and cell cycle processes | ||
Am J Physiol Cell Physiol Melatonin partially rescues defects induced by tranexamic acid exposure during oocyte maturation in mice |
