CoraLite®488-conjugated Alpha Tubulin Monoclonal antibody
Alpha Tubulin Monoclonal Antibody for FC (Intra), IF, WB
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, Canine
Applications
FC (Intra), IF, WB
Conjugate
CoraLite®488 Fluorescent Dye
CloneNo.
1E4C11
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HeLa cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IF detected in | HeLa cells, mouse embryo tissue |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:300-1:600 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
发表文章中的应用
IF | See 1 publications below |
产品信息
The immunogen of CL488-66031 is Alpha Tubulin Fusion Protein expressed in E. coli.
Tested Applications | FC (Intra), IF, WB |
Cited Applications | IF |
Tested Reactivity | human, mouse, rat, Canine |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Alpha Tubulin fusion protein Ag18034 |
Full Name | tubulin, alpha 1b |
Calculated molecular weight | 50 kDa |
Observed molecular weight | 50-55 kDa |
GenBank accession number | BC009314 |
Gene symbol | TUBA1B |
Uniprot | P68363 | Gene ID (NCBI) | 10376 |
Conjugate | CoraLite®488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 488 nm / 515 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS buffer with 50% glycerol, pH7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.
实验方案
Product Specific Protocols | |
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WB protocol for CL488 Alpha Tubulin antibody CL488-66031 | Download protocol |
IF protocol for CL488 Alpha Tubulin antibody CL488-66031 | Download protocol |
FC protocol for CL488 Alpha Tubulin antibody CL488-66031 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Transl Androl Urol Novel bi-allelic mutations in DNAH1 cause multiple morphological abnormalities of the sperm flagella resulting in male infertility. | ||
Clin Genet A novel homozygous CFAP65 mutation in humans causes male infertility with multiple morphological abnormalities of the sperm flagella. |