CoraLite®594-conjugated Alpha Tubulin Monoclonal antibody

Alpha Tubulin Monoclonal Antibody for FC, IF, WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, Canine

Applications

FC, IF, WB

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E4C11

Cat No : CL594-66031

Print datasheet

Synonyms

Alpha tubulin ubiquitous, alpha tubulin,a tubulin, K ALPHA 1, TUBA1B, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b, α-tubulin



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66031-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

发表文章中的应用

IFSee 2 publications below

产品信息

The immunogen of CL594-66031 is Alpha Tubulin Fusion Protein expressed in E. coli.

Tested Applications FC, IF, WB
Cited Applications IF
Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman, mouse
Immunogen Alpha Tubulin fusion protein Ag18034 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tubulin, alpha 1b
Synonyms Alpha tubulin ubiquitous, alpha tubulin,a tubulin, K ALPHA 1, TUBA1B, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b, α-tubulin
Calculated molecular weight 50 kDa
GenBank accession numberBC009314
Gene symbol TUBA1B
UniprotP68363
Gene ID (NCBI) 10376
RRIDAB_2883483
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm / 614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

实验方案

Product Specific Protocols
WB protocol for CL594 Alpha Tubulin antibody CL594-66031Download protocol
IF protocol for CL594 Alpha Tubulin antibody CL594-66031Download protocol
FC protocol for CL594 Alpha Tubulin antibody CL594-66031Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Exp Mol Med

Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes.

Authors - Long-Fei Wu
mouseIF

Biology (Basel)

Effects of IGF-1 on the Three-Dimensional Culture of Ovarian Preantral Follicles and Superovulation Rates in Mice.

Authors - Shizhen Dai