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Lamin A/C Polyclonal antibody
Lamin A/C Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)
Conjugate
Unconjugated
216
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HEK-293 cells, HUVEC cells, mouse ovary tissue, SKOV-3 cells, HeLa cells, A375 cells, C6 cells, NIH/3T3 cells |
Positive IP detected in | HeLa cells, A375 cells |
Positive IHC detected in | mouse heart tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Positive FC (Intra) detected in | HEK-293T cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10298-1-AP targets Lamin A/C in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, monkey, duck |
Immunogen | Lamin A/C fusion protein Ag0408 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | lamin A/C |
Synonyms | LaminA/C, lamin A, Prelamin-A/C, LMNA, LMN1 |
Calculated Molecular Weight | 65 kDa |
Observed Molecular Weight | 65 kDa, 70 kDa |
GenBank Accession Number | BC003162 |
Gene Symbol | Lamin A/C |
Gene ID (NCBI) | 4000 |
ENSEMBL Gene ID | ENSG00000160789 |
RRID | AB_2296961 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P02545 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Lamin A/C antibody 10298-1-AP | Download protocol |
IHC protocol for Lamin A/C antibody 10298-1-AP | Download protocol |
IF protocol for Lamin A/C antibody 10298-1-AP | Download protocol |
IP protocol for Lamin A/C antibody 10298-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer | ||
Nat Microbiol Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus. | ||
Mol Cell Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells. | ||
J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. | ||
Cancer Cell SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis. | ||
Sci Adv XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility |