验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HeLa cells, NIH/3T3 cells, HSC-T6 cells |
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80713-1-PBS
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL750-80713 targets Beta Tubulin in WB applications and shows reactivity with human, mouse, rat, zebrafish samples.
经测试应用 | WB Application Description |
经测试反应性 | human, mouse, rat, zebrafish |
免疫原 | Beta Tubulin fusion protein Ag0117 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | tubulin, beta 3 |
别名 | Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4 |
计算分子量 | 450 aa, 50 kDa |
观测分子量 | 50-55 kDa |
GenBank蛋白编号 | BC000748 |
基因名称 | TUBB3 |
Gene ID (NCBI) | 10381 |
RRID | AB_3086573 |
偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
最大激发/发射波长 | 755 nm / 780 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q13509 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for CL Plus 750 Beta Tubulin antibody CL750-80713 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |