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验证数据展示

  • WB analysis using HRP-80713

    WB analysis using HRP-80713

    Various lysates were subjected to SDS PAGE followed by western blot with HRP-80713 (Beta Tubulin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

HRP-conjugated Beta Tubulin Recombinant antibody

Beta Tubulin Uni-rAbTM Recombinant Antibody for WB
Cat No. HRP-80713

Print datasheet

CloneNo. 2O13

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB

Synonyms

Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  HRP
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

HRP-80713 targets Beta Tubulin in WB applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse, rat, zebrafish
Immunogen Beta Tubulin fusion protein Ag0117 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tubulin, beta 3
Synonyms Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC000748
Gene Symbol TUBB3
Gene ID (NCBI) 10381
RRIDAB_3673981
Conjugate HRP
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13509
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

实验方案

Product Specific Protocols
WB protocol for HRP Beta Tubulin antibody HRP-80713Download protocol
Standard Protocols
Click here to view our Standard Protocols