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- KD/KO Validated
VDAC1/Porin Polyclonal antibody
VDAC1/Porin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
FC, IF, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells, rat liver tissue, HepG2 cells, human skeletal muscle tissue, HEK-293 cells, HeLa cells, mouse liver tissue, A431 cells, mouse heart tissue, ROS1728 cells, mouse brain tissue, rat brain tissue, rat heart tissue, NIH/3T3 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human heart tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human liver cancer tissue |
| Positive FC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
产品信息
55259-1-AP targets VDAC1/Porin in WB, PLA, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF, IHC, IP, WB, ELISA |
| Cited Applications | CoIP, FC, IF, IHC, IP, PLA, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, hamster, monkey, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | voltage-dependent anion channel 1 |
| Calculated molecular weight | 31 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | NM_003374 |
| Gene symbol | VDAC1 |
| Uniprot | P21796 | Gene ID (NCBI) | 7416 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VDAC1/Porin antibody 55259-1-AP | Download protocol |
| IHC protocol for VDAC1/Porin antibody 55259-1-AP | Download protocol |
| IF protocol for VDAC1/Porin antibody 55259-1-AP | Download protocol |
| IP protocol for VDAC1/Porin antibody 55259-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Eur J Pharmacol A novel ent-kaurane diterpenoid analog, DN3, selectively kills human gastric cancer cells via acting directly on mitochondria. | ||
EBioMedicine DsbA-L ameliorates high glucose induced tubular damage through maintaining MAM integrity. | ||
Cells Perilipin 5 Protects against Cellular Oxidative Stress by Enhancing Mitochondrial Function in HepG2 Cells. | ||
Mol Genet Metab Rep Neonatal mitochondrial hepatoencephalopathy caused by novel GFM1 mutations. | ||
J Cell Mol Med Novel mitochondrion-targeting copper(II) complex induces HK2 malfunction and inhibits glycolysis via Drp1-mediating mitophagy in HCC. |
