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VDAC1/Porin Polyclonal antibody 40 Publications

Rabbit Polyclonal| Catalog number: 55259-1-AP

Featured Product KD/KO validated

浓度: 700 μg/ml

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Applications

Tested Applications:
FC, IHC, IP, WB,ELISA
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Cited Applications:
CoIP, IHC, PLA, WB


Images See all

WB analysis of HeLa cells using 55259-1-AP

WB result of VDAC1 antibody (55259-1-AP, 1:2000) with si-Control and si-VDAC1 transfected HeLa cells.

WB analysis using 55259-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IHC staining of human heart using 55259-1-AP

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IHC staining of human heart using 55259-1-AP

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FC experiment of HepG2 using 55259-1-AP

1X10^6 HepG2 cells were stained with 0.2ug VDAC1/Porin antibody (55259-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

IP experiment of A431 using 55259-1-AP

IP Result of anti-VDAC1/Porin (IP:55259-1-AP, 4ug; Detection:55259-1-AP 1:500) with A431 cells lysate 2400ug.

Species Specificity:
human, mouse, rat
Cited Species:
hamster, human, monkey, mouse, pig, rat
Positive WB detected in:
RAW 264.7 cells, A431 cells, HEK-293 cells, HeLa cells, HepG2 cells, human skeletal muscle tissue, mouse heart tissue, mouse liver tissue, rat liver tissue, ROS1728 cells, mouse brain tissue, rat brain tissue, NIH/3T3 cells, rat heart tissue
Positive IP detected in:
A431 cells
Positive IHC detected in:
human heart tissue, human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:1000-1:6000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
voltage-dependent anion channel 1
Calculated molecular weight:
31 kDa
Observed molecular weight:
31 kDa
GenBank accession number:
NM_003374
Gene ID (NCBI):
7416
Gene symbol
VDAC1
Synonyms
hVDAC1, Plasmalemmal porin, Porin, PORIN 31 HL, Porin 31HL, Porin 31HM, VDAC, VDAC 1, VDAC1

Background

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.

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