验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells, rat liver tissue, HepG2 cells, human skeletal muscle tissue, HEK-293 cells, HeLa cells, mouse liver tissue, A431 cells, mouse heart tissue, ROS1728 cells, mouse brain tissue, rat brain tissue, rat heart tissue, NIH/3T3 cells |
| Positive IHC detected in | human heart tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
| Positive FC (Intra) detected in | HepG2 cells |
This antibody is not recommended for immunocytofluorescent assays.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55259-1-AP targets VDAC1/Porin in WB, IHC, IF-P, FC (Intra), CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP, PLA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, canine, monkey, chicken, bovine, hamster, duck |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | voltage-dependent anion channel 1 |
| 别名 | Porin, VDAC1, hVDAC1, Plasmalemmal porin, PORIN 31 HL |
| 计算分子量 | 31 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | NM_003374 |
| 基因名称 | Porin |
| Gene ID (NCBI) | 7416 |
| RRID | AB_10837225 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P21796 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VDAC1/Porin antibody 55259-1-AP | Download protocol |
| IHC protocol for VDAC1/Porin antibody 55259-1-AP | Download protocol |
| IF protocol for VDAC1/Porin antibody 55259-1-AP | Download protocol |
| FC protocol for VDAC1/Porin antibody 55259-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell IRGQ-mediated autophagy in MHC class I quality control promotes tumor immune evasion | ||
Nat Commun MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration | ||
Mol Cell Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology | ||
Nat Commun An ESCRT-dependent step in fatty acid transfer from lipid droplets to mitochondria through VPS13D-TSG101 interactions. | ||
Nucleic Acids Res The human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases. | ||
Mol Biol Evol Functional Attenuation of UCP1 as the Potential Mechanism for Thickened Blubber Layer in Cetaceans |
