HRP-conjugated GST Tag Monoclonal antibody
GST Tag Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2a
Reactivity
recombinant protein, schistosoma japonicum and More (1)
Applications
WB and More (1)
Conjugate
HRP Fluorescent Dye
验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| Sample-dependent, check data in validation data gallery | |
产品信息
HRP-66001 targets GST Tag in WB, IP applications and shows reactivity with recombinant protein, schistosoma japonicum samples.
| Tested Applications | WB |
| Cited Applications | IP, WB |
| Tested Reactivity | recombinant protein, schistosoma japonicum |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein |
| Full Name | GST Tag |
| Calculated molecular weight | 26 kDa |
| Observed molecular weight | - |
| GenBank accession number | FN315687 |
| Gene symbol | |
| Gene ID (NCBI) | |
| Conjugate | HRP Fluorescent Dye |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins. This antibody is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
发表文章
| Species | Application | Title |
|---|---|---|
J Immunol Res TNPO1-Mediated Nuclear Import of FUBP1 Contributes to Tumor Immune Evasion by Increasing NRP1 Expression in Cervical Cancer. | ||
Nat Metab The SESAME complex regulates cell senescence through the generation of acetyl-CoA. | ||
Cell Death Dis HERC3 regulates epithelial-mesenchymal transition by directly ubiquitination degradation EIF5A2 and inhibits metastasis of colorectal cancer. | ||
Virus Genes The host cellular protein Ndufaf4 interacts with the vesicular stomatitis virus M protein and affects viral propagation. | ||
Vet Microbiol The matrix protein of vesicular stomatitis virus inhibits host-directed transcription of target genes via interaction with the TFIIH subunit p8. | ||
Vet Microbiol Host factor cyclophilin B affects Orf virus replication by interacting with viral ORF058 protein. |
