GST Tag Monoclonal antibody
GST Tag Monoclonal Antibody for IP, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Recombinant protein, Schistosoma japonicum and More (3)
Applications
IP, WB, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
3G12B10
验证数据展示
经过测试的应用
Positive WB detected in | Recombinant protein |
Positive IP detected in | E. coli lysate |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:5000-1:20000 for WB |
Sample-dependent, check data in validation data gallery |
产品信息
66001-2-Ig targets GST Tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with Recombinant protein, Schistosoma japonicum samples.
Tested Applications | IP, WB, ELISA |
Cited Applications | CoIP, IF, IP, WB |
Tested Reactivity | Recombinant protein, Schistosoma japonicum |
Cited Reactivity | human, mouse, pig |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Recombinant protein |
Full Name | GST Tag |
Calculated molecular weight | 26 kDa |
GenBank accession number | FN315687 |
Gene symbol | |
Gene ID (NCBI) | |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the N- or C-terminus of targeted proteins.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for GST Tag antibody 66001-2-Ig | Download protocol |
IP protocol for GST Tag antibody 66001-2-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
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Dev Cell FGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver Tumorigenesis. | ||
MBio Nonmuscle Myosin Heavy Chain IIA Recognizes Sialic Acids on Sialylated RNA Viruses To Suppress Proinflammatory Responses via the DAP12-Syk Pathway. | ||
Acta Pharmacol Sin Inhibition of AXL enhances chemosensitivity of human ovarian cancer cells to cisplatin via decreasing glycolysis. | ||
Cell Rep Regulation of NMDA receptor trafficking and gating by activity-dependent CaMKIIα phosphorylation of the GluN2A subunit. |