GST Tag Monoclonal antibody

GST Tag Monoclonal Antibody for IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Recombinant protein, Schistosoma japonicum and More (3)

Applications

IP, WB, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

3G12B10

Cat No : 66001-2-Ig

Print datasheet

Synonyms

GST, GST Tag



经过测试的应用

Positive WB detected inRecombinant protein
Positive IP detected inE. coli lysate

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:20000 for WB
Sample-dependent, check data in validation data gallery

产品信息

66001-2-Ig targets GST Tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with Recombinant protein, Schistosoma japonicum samples.

Tested Applications IP, WB, ELISA
Cited Applications CoIP, IF, IP, WB
Tested Reactivity Recombinant protein, Schistosoma japonicum
Cited Reactivityhuman, mouse, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name GST Tag
Calculated molecular weight 26 kDa
GenBank accession numberFN315687
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the N- or C-terminus of targeted proteins.

实验方案

Product Specific Protocols
WB protocol for GST Tag antibody 66001-2-IgDownload protocol
IP protocol for GST Tag antibody 66001-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Dev Cell

FGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver Tumorigenesis.

Authors - Suyuan Ji

MBio

Nonmuscle Myosin Heavy Chain IIA Recognizes Sialic Acids on Sialylated RNA Viruses To Suppress Proinflammatory Responses via the DAP12-Syk Pathway.

Authors - Yingqi Liu

EBioMedicine

Bach1-induced suppression of angiogenesis is dependent on the BTB domain.

Authors - Li Jiang
WB,IP

Acta Pharmacol Sin

Inhibition of AXL enhances chemosensitivity of human ovarian cancer cells to cisplatin via decreasing glycolysis.

Authors - Min Tian
WB

Cell Rep

PICK1 Controls Activity-Dependent Synaptic Vesicle Cargo Retrieval.

Authors - Xuan Ling Hilary Yong
WB

Cell Rep

Regulation of NMDA receptor trafficking and gating by activity-dependent CaMKIIα phosphorylation of the GluN2A subunit.

Authors - Xuan Ling Hilary Yong