验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
| Positive IP detected in | E. coli lysate |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66001-2-Ig targets GST Tag in WB, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with Recombinant protein, Schistosoma japonicum samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IF, IP, CoIP, ChIP |
| Tested Reactivity | Recombinant protein, Schistosoma japonicum |
| Cited Reactivity | human |
| Immunogen | Recombinant protein 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | GST Tag |
| Synonyms | GST, GST Tag |
| Calculated Molecular Weight | 26 kDa |
| GenBank Accession Number | FN315687 |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881488 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the N- or C-terminus of targeted proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GST Tag antibody 66001-2-Ig | Download protocol |
| IP protocol for GST Tag antibody 66001-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
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