mCherry Polyclonal antibody

mCherry Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (6)

Applications

WB, IF/ICC, ELISA and More (4)

Conjugate

Unconjugated

Cat no : 26765-1-AP

Print datasheet

Synonyms

mCherry, Red fluorescence protein, red fluorescent protein, RFP, RFP-tag



经过测试的应用

Positive WB detected inTransfected HEK-293 cells
Positive IF/ICC detected inTransfected HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26765-1-AP targets mCherry in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, zebrafish, yeast, plant, d. punctatus
Immunogen mCherry fusion protein Ag25320 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mCherry
Synonyms mCherry, Red fluorescence protein, red fluorescent protein, RFP, RFP-tag
Calculated Molecular Weight 27 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2876881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages.

The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm).

When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a rabbit polyclonal antibody raised against mCherry. It can be used to detect mCherry, dsRed, tdTomato, and mScarlet.


实验方案

Product Specific Protocols
WB protocol for mCherry antibody 26765-1-APDownload protocol
IF protocol for mCherry antibody 26765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nat Commun

TBC1D23 mediates Golgi-specific LKB1 signaling

Authors - Yingfeng Tu
humanWB

Nat Commun

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Authors - Ji Geng
humanIF

J Extracell Vesicles

Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion

Authors - Chuqi Liu
humanIP

Nat Commun

DELE1 tracks perturbed protein import and processing in human mitochondria.

Authors - Evelyn Fessler
humanIF

EMBO J

GRAMD1/ASTER-mediated cholesterol transport promotes Smoothened cholesterylation at the endoplasmic reticulum

Authors - Zhi-Ping Qiu
humanIP

Protein Cell

cGAS guards against chromosome end-to-end fusions during mitosis and facilitates replicative senescence.

Authors - Xiaocui Li