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YY1 Polyclonal antibody 16 Publications

Rabbit Polyclonal| Catalog number: 22156-1-AP

Featured Product KD/KO validated

浓度: 500 μg/ml

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Applications

Tested Applications:
IF, IHC, IP, WB,ELISA
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Cited Applications:
chIP, IF, IHC, IP, WB


Images See all

IHC staining of human breast cancer using 22156-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22156-1-AP (YY1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IF Staining of HepG2 using 22156-1-AP

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 22156-1-AP (YY1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

WB analysis of A549 cells using 22156-1-AP

A549 cells (shcontrol and shRNA of YY1) were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000.

WB analysis of Jurkat using 22156-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP experiment of HeLa using 22156-1-AP

IP Result of anti-YY1 (IP:22156-1-AP, 5ug; Detection:22156-1-AP 1:700) with HeLa cells lysate 880ug.

Species Specificity:
human, mouse
Cited Species:
bovine, human, mouse, pig, rat
Positive WB detected in:
Jurkat cells, A549 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
YY1 fusion protein Ag17792
Full name:
YY1 transcription factor
Calculated molecular weight:
414 aa, 45 kDa
Observed molecular weight:
65-70 kDa
GenBank accession number:
BC037308
Gene ID (NCBI):
7528
Gene symbol
YY1
Synonyms
DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor

Background

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. This is a rabbit polyclonal antibody raised against full length YY1 of human origin.

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