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- KD/KO Validated
YY1 Polyclonal antibody
YY1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
IF, IHC, IP, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, A549 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
产品信息
The immunogen of 22156-1-AP is YY1 Fusion Protein expressed in E. coli.
| Tested Applications | IF, IHC, IP, WB,ELISA |
| Cited Applications | chIP, IF, IHC, IP, WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, bovine, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | YY1 fusion protein Ag17792 |
| Full Name | YY1 transcription factor |
| Calculated molecular weight | 414 aa, 45 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC037308 |
| Gene symbol | YY1 |
| Uniprot | P25490 | Gene ID (NCBI) | 7528 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. This is a rabbit polyclonal antibody raised against full length YY1 of human origin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for YY1 antibody 22156-1-AP | Download protocol |
| IHC protocol for YY1 antibody 22156-1-AP | Download protocol |
| IF protocol for YY1 antibody 22156-1-AP | Download protocol |
| IP protocol for YY1 antibody 22156-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun YY1-regulated LINC00152 promotes triple negative breast cancer progression by affecting on stability of PTEN protein. | ||
Anim Genet A haplotype variant of Hu sheep follicle-stimulating hormone receptor promoter region decreases transcriptional activity. | ||
Ann Transl Med Heat shock cognate protein 70 promotes the differentiation of C2C12 myoblast and targets Yin Yang 1. | ||
Cell Death Dis Long non-coding RNA Xist regulates oocyte loss via suppressing miR-23b-3p/miR-29a-3p maturation and upregulating STX17 in perinatal mouse ovaries. | ||
Front Vet Sci Effect of Promoter Methylation on the Expression of Porcine MUC2 Gene and Resistance to PEDV Infection. | ||
Int J Oncol Upregulation of miR-146a by YY1 depletion correlates with delayed progression of prostate cancer.
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