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CoraLite® Plus 488-conjugated S1 tag Monoclonal antibody

S1 tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A9B5

Cat No : CL488-66165

Print datasheet

Synonyms

验证数据展示

  • WB analysis using CL488-66165

    WB analysis using CL488-66165

    S1 tagged protein lysate was subjected to SDS PAGE followed by western blot with CL488-66165 (S1 tag antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRecombinant protein
For other applications, we recommend the unconjugated version of this antibody, 66165-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66165 targets S1 tag in WB applications and shows reactivity with recombinant protein samples.

Tested Applications WB
Tested Reactivity recombinant protein
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name S1 tag
Synonyms
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S1 tag, with sequence NANNPDWDF, is derived from the hepatitis B virus preS1 region. S1 tag is widely used in various protein expression systems. This antibody can detect the over-expressed fusion proteins containing S1 epitope tag.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 S1 tag antibody CL488-66165Download protocol
Standard Protocols
Click here to view our Standard Protocols