TBP Polyclonal antibody

TBP Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Rat and More (3)

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 22006-1-AP

Print datasheet

Synonyms

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID



经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman breast cancer tissue, mouse testis tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22006-1-AP targets TBP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, Rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, Rat
Cited Reactivityhuman, mouse, rat, monkey, pig
Immunogen TBP fusion protein Ag12383 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TATA box binding protein
Synonyms GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID
Calculated Molecular Weight 338 aa, 38 kDa
Observed Molecular Weight mouse/rat 33-36 kDa and human 37-43kDa
GenBank Accession NumberBC110341
Gene Symbol TBP
Gene ID (NCBI) 6908
RRIDAB_10951514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20226
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.

实验方案

Product Specific Protocols
WB protocol for TBP antibody 22006-1-APDownload protocol
IHC protocol for TBP antibody 22006-1-APDownload protocol
IP protocol for TBP antibody 22006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Hepatology

Steatotic hepatocytes release mature VLDL via methionine and tyrosine metabolism in a Keap1-Nrf2 dependent manner.

Authors - Akitoshi Sano
humanWB

Nat Commun

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Authors - Gongwei Wu
humanWB

Elife

KPNB1 mediates PER/CRY nuclear translocation and circadian clock function.

Authors - Yool Lee
humanWB

J Hazard Mater

ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes.

Authors - Xuan Lai
humanWB

Cell Death Dis

4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer

Authors - Yanan Wang
humanWB

EMBO Rep

LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.

Authors - Xuedan Sun