TBP Polyclonal antibody

TBP Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (3)


IHC, IP, WB,ELISA and More (1)



Cat No : 22006-1-AP

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GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID


Positive WB detected inCOLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery


The immunogen of 22006-1-AP is TBP Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB,ELISA
Cited Applications IF, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, monkey, pig
Immunogen TBP fusion protein Ag12383 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TATA box binding protein
Synonyms GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID
Calculated molecular weight 338 aa, 38 kDa
Observed molecular weight mouse/rat 33-36 kDa and human 37-43kDa
GenBank accession numberBC110341
Gene symbol TBP
Gene ID (NCBI) 6908
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.


Product Specific Protocols
WB protocol for TBP antibody 22006-1-APDownload protocol
IHC protocol for TBP antibody 22006-1-APDownload protocol
IP protocol for TBP antibody 22006-1-APDownload protocol
Standard Protocols
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