|Positive WB detected in||COLO 320 cells|
|Positive IP detected in||COLO 320 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22006-1-AP is TBP Fusion Protein expressed in E. coli.
|Tested Applications||IHC, IP, WB,ELISA|
|Cited Applications||IF, WB|
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TBP fusion protein Ag12383|
|Full Name||TATA box binding protein|
|Calculated molecular weight||338 aa, 38 kDa|
|Observed molecular weight||mouse/rat 33-36 kDa and human 37-43kDa|
|GenBank accession number||BC110341|
|Uniprot||P20226||Gene ID (NCBI)||6908|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.
Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4.
Front Cell Neurosci
IRAK-M Deficiency Exacerbates Ischemic Neurovascular Injuries in Experimental Stroke Mice.
Am J Physiol Cell Physiol
Expansion of p63-positive CCSP-positive epithelial cells and altered expressions of p63 isoforms in the lung as novel features of aging.
Ets1-Mediated Acetylation of FoxO1 Is Critical for Gluconeogenesis Regulation during Feed-Fast Cycles.
A novel function of anaphase promoting complex subunit 10 in tumor progression in non-small cell lung cancer.
Interleukin-1 receptor associated kinase (IRAK)-M -mediated type 2 microglia polarization ameliorates the severity of experimental autoimmune encephalomyelitis (EAE).