V5-tag Polyclonal antibody

V5-tag Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (3)

Applications

IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14440-1-AP

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Synonyms

GKPIPNPLLGLDST, V5, V5 tag, V5-tag



经过测试的应用

Positive WB detected inRecombinant protein
Positive IP detected inRecombinant protein protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
Sample-dependent, check data in validation data gallery

产品信息

14440-1-AP targets V5-tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications IP, WB, ELISA
Cited Applications IF, IP, WB
Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name V5-tag
Calculated molecular weight 1 kDa
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.

实验方案

Product Specific Protocols
WB protocol for V5-tag antibody 14440-1-APDownload protocol
IP protocol for V5-tag antibody 14440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cell Cycle

A novel function of anaphase promoting complex subunit 10 in tumor progression in non-small cell lung cancer.

Authors - Yanan Wang

J Exp Clin Cancer Res

FKBP9 promotes the malignant behavior of glioblastoma cells and confers resistance to endoplasmic reticulum stress inducers.

Authors - Huizhe Xu
WB

Fish Shellfish Immunol

Identification and characterization of an apoptosis-inducing factor 1 involved in apoptosis and immune defense of oyster, Crassostrea gigas.

Authors - Xue Qiao
WB

J Med Virol

SARS-CoV-2 ORF9b antagonizes type I and III interferons by targeting multiple components of the RIG-I/MDA-5-MAVS, TLR3-TRIF, and cGAS-STING signaling pathways.

Authors - Lulu Han
WB

J Gastrointest Oncol

N-myc downstream-regulated gene 2 promotes the protein stability of estrogen receptor beta via inhibition of ubiquitin-protein ligase E3A to suppress colorectal cancer.

Authors - Jun Zhu

Front Cell Infect Microbiol

Rv3722c Promotes Mycobacterium tuberculosis Survival in Macrophages by Interacting With TRAF3.

Authors - Yingying Lei