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Conjugate
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Conjugate
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V5-tag Polyclonal antibody

V5-tag Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (2)

Applications

IP, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 14440-1-AP

Print datasheet

Synonyms

GKPIPNPLLGLDST, V5, V5 tag, V5-tag

验证数据展示

  • WB analysis of Recombinant protein using 14440-1-AP

    WB analysis of Recombinant protein using 14440-1-AP

    Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:32000 incubated at room temperature for 1.5 hours.

  • IP experiment of Recombinant protein using 14440-1-AP

    IP experiment of Recombinant protein using 14440-1-AP

    IP result of anti-V5-tag (IP:14440-1-AP, 3ug; Detection:14440-1-AP 1:20000) with Recombinant protein protein lysate 3000ug.

经过测试的应用

Positive WB detected inRecombinant protein
Positive IP detected inRecombinant protein protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14440-1-AP targets V5-tag in WB,IP,IF,RIP,ChIP,ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB,IP,IF,RIP,ChIP
Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name V5-tag
Synonyms GKPIPNPLLGLDST, V5, V5 tag, V5-tag
Calculated Molecular Weight 1 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2878059
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.

实验方案

Product Specific Protocols
WB protocol for V5-tag antibody 14440-1-APDownload protocol
IP protocol for V5-tag antibody 14440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

J Extracell Vesicles

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

Authors - Xiaohan Gao
View Article
WB

Sci Adv

The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals

Authors - Liuke Sun
View Article
IF

Nat Commun

Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.

Authors - Xianding Sun
View Article
WB

Nucleic Acids Res

HSP90 and Aha1 modulate microRNA maturation through promoting the folding of Dicer1.

Authors - Xiaochuan Liu
View Article
humanIP,WB

Nucleic Acids Res

CGG repeats trigger translational frameshifts that generate aggregation-prone chimeric proteins.

Authors - Shannon E Wright
View Article
WB

Cell Death Differ

Mitophagy protein PINK1 suppresses colon tumor growth by metabolic reprogramming via p53 activation and reducing acetyl-CoA production.

Authors - Kunlun Yin
View Article