验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
| Positive IP detected in | Recombinant protein protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14440-1-AP targets V5-tag in WB, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP, ChIP, RIP |
| 经测试反应性 | recombinant protein |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | V5 Tag |
| 别名 | GKPIPNPLLGLDST, V5, V5 tag, V5-tag |
| 计算分子量 | 1 kDa |
| 基因名称 | V5 Tag |
| Gene ID (NCBI) | 4 |
| RRID | AB_2878059 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for V5-tag antibody 14440-1-AP | Download protocol |
| IP protocol for V5-tag antibody 14440-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation | ||
J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. | ||
Nat Chem Biol E2-Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells | ||
Acta Pharm Sin B Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis | ||
Sci Adv The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals | ||
Nat Commun Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway. |
