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  • Featured Product
  • KD/KO Validated

Lamin A/C Recombinant antibody

Lamin A/C Recombinant Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

5I16

Cat No : 81042-1-RR

Print datasheet

Synonyms

70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, lamin A, lamin A/C, LDP1, LFP, LGMD1B, LMN1, LMNA, LMNC, Prelamin A/C, PRO1

验证数据展示

  • WB analysis using 81042-1-RR

    WB analysis using 81042-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81042-1-RR (Lamin A/C antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 81042-1-RR

    WB analysis of HeLa using 81042-1-RR

    WB result of Lamin A/C antibody (81042-1-RR; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin A/C transfected HeLa cells.

  • IHC staining of mouse heart using 81042-1-RR

    IHC staining of mouse heart using 81042-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Lamin A/C antibody included
  • IHC staining of mouse heart using 81042-1-RR

    IHC staining of mouse heart using 81042-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Lamin A/C antibody included
  • IF Staining of HepG2 using 81042-1-RR

    IF Staining of HepG2 using 81042-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Lamin A/C antibody (81042-1-RR, Clone: 5I16 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, RAW 264.7 cells, HeLa cells, HUVEC cells, A431 cells, SKOV-3 cells, HSC-T6 cells, NIH/3T3 cells, C6 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Lamin A/C Ready-To-Use IHC Kit. Lamin A/C primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81042-1-RR targets Lamin A/C in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen Lamin A/C fusion protein Ag0408 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lamin A/C
Synonyms 70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, lamin A, lamin A/C, LDP1, LFP, LGMD1B, LMN1, LMNA, LMNC, Prelamin A/C, PRO1
Calculated Molecular Weight 65 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC003162
Gene Symbol LMNA
Gene ID (NCBI) 4000
ENSEMBL Gene IDENSG00000160789
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02545
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.

实验方案

Product Specific Protocols
WB protocol for Lamin A/C antibody 81042-1-RRDownload protocol
IHC protocol for Lamin A/C antibody 81042-1-RRDownload protocol
IF protocol for Lamin A/C antibody 81042-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols