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验证数据展示

  • WB analysis using 81042-1-RR (same clone as 81042-1-PBS)

    WB analysis using 81042-1-RR (same clone as 81042-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81042-1-RR (Lamin A/C antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81042-1-RR (same clone as 81042-1-PBS)

    WB analysis of HeLa using 81042-1-RR (same clone as 81042-1-PBS)

    WB result of Lamin A/C antibody (81042-1-RR; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin A/C transfected HeLa cells. This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 81042-1-RR (same clone as 81042-1-PBS)

    IHC staining of human colon using 81042-1-RR (same clone as 81042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:12000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Lamin A/C antibody included
  • IHC staining of human colon using 81042-1-RR (same clone as 81042-1-PBS)

    IHC staining of human colon using 81042-1-RR (same clone as 81042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:12000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Lamin A/C antibody included
  • IHC staining of mouse heart using 81042-1-RR (same clone as 81042-1-PBS)

    IHC staining of mouse heart using 81042-1-RR (same clone as 81042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Lamin A/C antibody included
  • IHC staining of mouse heart using 81042-1-RR (same clone as 81042-1-PBS)

    IHC staining of mouse heart using 81042-1-RR (same clone as 81042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Lamin A/C antibody included
  • IF Staining of HepG2 using 81042-1-RR (same clone as 81042-1-PBS)

    IF Staining of HepG2 using 81042-1-RR (same clone as 81042-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Lamin A/C antibody (81042-1-RR, Clone: 5I16 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81042-1-RR

    Affinity and Kinetic Characterization of 81042-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81042-1-RR against Human Lamin A/C were performed. The affinity constant is 92.9 pM.

  • Featured Product
  • KD/KO验证

Lamin A/C Recombinant antibody, PBS Only

Lamin A/C Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81042-1-PBS

产品说明书

CloneNo. 5I16

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

LaminA/C, LMNA, LMN1, Lamin-A/C, lamin A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Lamin A/C Ready-To-Use IHC Kit. Lamin A/C primary antibody included.

产品信息

81042-1-PBS targets Lamin A/C in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Lamin A/C fusion protein Ag0408 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lamin A/C
别名 LaminA/C, LMNA, LMN1, Lamin-A/C, lamin A
计算分子量 65 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC003162
基因名称 Lamin A/C
Gene ID (NCBI) 4000
ENSEMBL Gene IDENSG00000160789
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02545
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.