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Histone H3 Recombinant antibody

Histone H3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241079B2

Cat no : 81984-2-RR

Print datasheet

Synonyms

HIST2H3A, H3-clustered histone 14, H3-clustered histone 13, H3C15, H3C14

验证数据展示

  • WB analysis using 81984-2-RR

    WB analysis using 81984-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81984-2-RR (Histone H3 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 81984-2-RR

    IF Staining of HeLa using 81984-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Histone H3 antibody (81984-2-RR, Clone: 241079B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 81984-2-RR

    FC experiment of HepG2 using 81984-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Histone H3 Recombinant antibody (81984-2-RR, Clone:241079B2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 81984-2-RR

    Affinity and Kinetic Characterization of 81984-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81984-2-RR against Human Histone H3 were performed. The affinity constant is 12.2 nM.

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, mouse brain tissue, mouse kidney tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Histone-H3 Ready-To-Use IHC Kit. Histone-H3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 81984-2-RR is Histone H3 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Histone H3 fusion protein Ag10644 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 2, H3a
Synonyms HIST2H3A, H3-clustered histone 14, H3-clustered histone 13, H3C15, H3C14
Calculated Molecular Weight 136 aa, 15 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC015544
Gene Symbol Histone H3
Gene ID (NCBI) 333932
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ71DI3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.

实验方案

Product Specific Protocols
WB protocol for Histone H3 antibody 81984-2-RRDownload protocol
IF protocol for Histone H3 antibody 81984-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols