MBP-Tag Polyclonal antibody
MBP-Tag Polyclonal Antibody for WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein and More (3)
Applications
WB,ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | lane1: MBP-NOL6 64kd; lane2 MBP 42kd, Recombinant protein |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Sample-dependent, check data in validation data gallery |
产品信息
15089-1-AP targets MBP-Tag in WB, IHC, IF, ELISA applications and shows reactivity with recombinant protein samples.
Tested Applications | WB,ELISA |
Cited Applications | ELISA, IF, IHC, WB |
Tested Reactivity | recombinant protein |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MBP-Tag fusion protein Ag0942 |
Full Name | MBP-Tag |
Calculated molecular weight | 40 kDa |
Gene symbol | |
Gene ID (NCBI) | |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (Maltose binding protein) TAG in some expression systems.
发表文章
Species | Application | Title |
---|---|---|
World J Microbiol Biotechnol The Shigella type three secretion system effector OspF invades host nucleus by binding host importin α1. | ||
Pediatr Res Intrauterine inflammation induced white matter injury protection by fibrinogen-like protein 2 deficiency in perinatal mice. | ||
Behav Brain Res Anatomical and behavioral outcomes following a graded hemi-contusive cervical spinal cord injury model in mice | ||
J Integr Plant Biol Protein farnesylation negatively regulates brassinosteroid signaling via reducing BES1 stability in Arabidopsis thaliana. | ||
Plant Physiol A CCCH-type zinc finger nucleic acid-binding protein quantitatively confers resistance against rice bacterial blight disease. |