MBP-Tag Polyclonal antibody

MBP-Tag Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (3)

Applications

WB,ELISA and More (2)

Conjugate

Unconjugated

Cat No : 15089-1-AP

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Synonyms

Maltose Binding Protein, MBP, MBP Tag



经过测试的应用

Positive WB detected inlane1: MBP-NOL6 64kd; lane2 MBP 42kd, Recombinant protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

产品信息

15089-1-AP targets MBP-Tag in WB, IHC, IF, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications WB,ELISA
Cited Applications ELISA, IF, IHC, WB
Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MBP-Tag fusion protein Ag0942
Full Name MBP-Tag
Calculated molecular weight 40 kDa
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (Maltose binding protein) TAG in some expression systems.

发表文章

SpeciesApplicationTitle

World J Microbiol Biotechnol

The Shigella type three secretion system effector OspF invades host nucleus by binding host importin α1.

Authors - Hongmei Zhao
mouseIHC

Pediatr Res

Intrauterine inflammation induced white matter injury protection by fibrinogen-like protein 2 deficiency in perinatal mice.

Authors - Di Zhan
mouseIF

Behav Brain Res

Anatomical and behavioral outcomes following a graded hemi-contusive cervical spinal cord injury model in mice

Authors - Zucheng Huang

J Biophotonics

Photobiomodulation therapy for repeated closed head injury in rats.

Authors - Baocheng Yang
WB

J Integr Plant Biol

Protein farnesylation negatively regulates brassinosteroid signaling via reducing BES1 stability in Arabidopsis thaliana.

Authors - Zengxiu Feng
WB

Plant Physiol

A CCCH-type zinc finger nucleic acid-binding protein quantitatively confers resistance against rice bacterial blight disease.

Authors - Deng Hanqing H