验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, MDCK cells, NIH/3T3 cells, PC-12 cells, mouse heart tissue, rat heart tissue |
Positive IHC detected in | mouse colon tissue, human breast cancer tissue, human prostate cancer tissue, human skeletal muscle tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, A549 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:60000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26520-1-AP targets Vinculin in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, canine samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse, rat, canine |
文献引用反应性 | human, mouse, rat, pig, rabbit, canine |
免疫原 | Vinculin fusion protein Ag24942 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | vinculin |
别名 | CMD1W, Metavinculin, MV, MVCL, VCL |
计算分子量 | 1133 aa, 124 kDa |
观测分子量 | 124 kDa |
GenBank蛋白编号 | BC039174 |
基因名称 | Vinculin |
Gene ID (NCBI) | 7414 |
RRID | AB_2868558 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P18206 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Vinculin antibody 26520-1-AP | Download protocol |
IHC protocol for Vinculin antibody 26520-1-AP | Download protocol |
IF protocol for Vinculin antibody 26520-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab Electron transport chain inhibition increases cellular dependence on purine transport and salvage | ||
Blood Epigenetic Regulation of Non-canonical Menin Targets Modulates Menin Inhibitor Response in Acute Myeloid Leukemia | ||
ACS Nano Polyethylenimine-Modified Mesoporous Silica Nanoparticles Induce a Survival Mechanism in Vascular Endothelial Cells via Microvesicle-Mediated Autophagosome Release | ||
Adv Sci (Weinh) Highly Effective and Noninvasive Near-Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min. | ||