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验证数据展示

  • IF Staining of A549 using CL594-26520

    IF Staining of A549 using CL594-26520

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 Vinculin antibody (CL594-26520) at dilution of 1:200, CL488-phalloidin (green).

  • IF Staining of HepG2 using CL594-26520

    IF Staining of HepG2 using CL594-26520

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Vinculin antibody (CL594-26520) at dilution of 1:200, CL488-phalloidin (green).

CoraLite®594-conjugated Vinculin Polyclonal antibody

Vinculin Polyclonal Antibody for IF/ICC
Cat No. CL594-26520

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

VCL, MVCL, MV, Metavinculin, CMD1W

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells, A549 cells, HUVEC cells
For other applications, we recommend the unconjugated version of this antibody, 26520-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-26520 targets Vinculin in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen Vinculin fusion protein Ag24942 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vinculin
Synonyms VCL, MVCL, MV, Metavinculin, CMD1W
Calculated Molecular Weight 1133 aa, 124 kDa
Observed Molecular Weight 117 kDa
GenBank Accession NumberBC039174
Gene Symbol Vinculin
Gene ID (NCBI) 7414
RRIDAB_2919877
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18206
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

实验方案

Product Specific Protocols
IF protocol for CL594 Vinculin antibody CL594-26520Download protocol
Standard Protocols
Click here to view our Standard Protocols