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ATP1A1 Polyclonal antibody

ATP1A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14418-1-AP

Print datasheet

Synonyms

Na+/K+ ATPase 1, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1

验证数据展示

  • WB analysis using 14418-1-AP

    WB analysis using 14418-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Caco-2 using 14418-1-AP

    IF Staining of Caco-2 using 14418-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed Caco-2 cells using ATP1A1 antibody (14418-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver cancer using 14418-1-AP

    IHC staining of human liver cancer using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ATP1A1 antibody included
  • IHC staining of human ovary tumor using 14418-1-AP

    IHC staining of human ovary tumor using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP1A1 antibody included
  • FC experiment of HEK-293 using 14418-1-AP

    FC experiment of HEK-293 using 14418-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ATP1A1 Polyclonal antibody (14418-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 14418-1-AP

    WB analysis using 14418-1-AP

    37°C incubated mouse brain total extract lysate and membrane extract lysate were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14418-1-AP

    WB analysis using 14418-1-AP

    37°C incubated HEK-293 membrane extract lysate were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 14418-1-AP

    IP experiment of HEK-293 using 14418-1-AP

    IP result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:20000) with HEK-293 cells lysate 1470 ug.

  • IHC staining of human cervical cancer using 14418-1-AP

    IHC staining of human cervical cancer using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP1A1 antibody included
  • IHC staining of human liver cancer using 14418-1-AP

    IHC staining of human liver cancer using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ATP1A1 antibody included
  • IHC staining of human ovary tumor using 14418-1-AP

    IHC staining of human ovary tumor using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP1A1 antibody included
  • IHC staining of human colon using 14418-1-AP

    IHC staining of human colon using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP1A1 antibody included
  • IHC staining of human colon using 14418-1-AP

    IHC staining of human colon using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP1A1 antibody included
  • IF Staining of HEK-293 using 14418-1-AP

    IF Staining of HEK-293 using 14418-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ATP1A1 antibody (14418-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse heart tissue, HEK-293 cells, 37°C incubated HEK-293 cells, 37°C incubated mouse brain tissue, rat heart tissue
Positive IP detected inHEK-293 cells, A431 cells
Positive IHC detected inhuman ovary tumor tissue, human cervical cancer tissue, human colon tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells, HEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

For optimal WB detection with 14418-1-AP, we do not recommend boiling the sample after lysis.

Planning an IHC experiment? We recommend our IHCeasy ATP1A1 Ready-To-Use IHC Kit. ATP1A1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14418-1-AP targets ATP1A1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, chicken
Immunogen ATP1A1 fusion protein Ag5763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Synonyms Na+/K+ ATPase 1, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1
Calculated Molecular Weight 113 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC050359
Gene Symbol ATP1A1
Gene ID (NCBI) 476
ENSEMBL Gene IDENSG00000163399
RRIDAB_2227873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05023
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

实验方案

Product Specific Protocols
WB protocol for ATP1A1 antibody 14418-1-APDownload protocol
IHC protocol for ATP1A1 antibody 14418-1-APDownload protocol
IF protocol for ATP1A1 antibody 14418-1-APDownload protocol
IP protocol for ATP1A1 antibody 14418-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nature

Low-dose metformin targets the lysosomal AMPK pathway through PEN2.

Authors - Teng Ma
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mouseWB

Immunity

Disruption of the Na+/K+-ATPase-purinergic P2X7 receptor complex in microglia promotes stress-induced anxiety

Authors - Songqiang Huang
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WB

Adv Mater

Ultrasound Imaging of Tumor Vascular CD93 with MMRN2 Modified Microbubbles for Immune Microenvironment Prediction

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mouseWB

ACS Nano

Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer

Authors - Lingtong Meng
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WB

Adv Sci (Weinh)

Ultrasound Responsive Nanovaccine Armed with Engineered Cancer Cell Membrane and RNA to Prevent Foreseeable Metastasis

Authors - Wenqi Sun
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humanWB

Theranostics

Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer

Authors - Chen Li
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