• Featured Product
  • KD/KO Validated

ATP1A1 Polyclonal antibody

ATP1A1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 14418-1-AP

Print datasheet

Synonyms

ATP1A1, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha 1 subunit, Na+/K+ ATPase 1, Sodium potassium ATPase alpha 1 polypeptide, Sodium pump subunit alpha 1



经过测试的应用

Positive WB detected inmouse heart tissue, HEK-293 cells
Positive IP detected inHEK-293 cells, A431 cells
Positive IHC detected inhuman liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCaco-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:400
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

产品信息

14418-1-AP targets ATP1A1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP1A1 fusion protein Ag5763
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated molecular weight 113 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC050359
Gene symbol ATP1A1
UniprotP05023
Gene ID (NCBI) 476
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

实验方案

Product Specific Protocols
WB protocol for ATP1A1 antibody 14418-1-APDownload protocol
IHC protocol for ATP1A1 antibody 14418-1-APDownload protocol
IF protocol for ATP1A1 antibody 14418-1-APDownload protocol
IP protocol for ATP1A1 antibody 14418-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Biomed Pharmacother

CP-25 combined with MTX/ LEF ameliorates the progression of adjuvant-induced arthritis by the inhibition on GRK2 translocation.

Authors - Xuezhi Yang
humanWB

Aging (Albany NY)

Laminin α4 overexpression in the anterior lens capsule may contribute to the senescence of human lens epithelial cells in age-related cataract.

Authors - Yu Yan
mouseWB

Cell Death Dis

CXCR7 regulates epileptic seizures by controlling the synaptic activity of hippocampal granule cells.

Authors - Tao Xu
mouseWB

Cells

GRK2 Mediated Abnormal Transduction of PGE2-EP4-cAMP-CREB Signaling Induces the Imbalance of Macrophages Polarization in Collagen-Induced Arthritis Mice.

Authors - Xuezhi Yang
humanWB

Front Oncol

The Binding of PD-L1 and Akt Facilitates Glioma Cell Invasion Upon Starvation via Akt/Autophagy/F-Actin Signaling.

Authors - Ruo Qiao Chen
mouseWB

J Diabetes Res

Identification of Protein Kinase C Isoforms Involved in Type 1 Diabetic Encephalopathy in Mice.

Authors - Jiayin Zheng