COXIV Monoclonal antibody
COXIV Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human and More (2)
Applications
FC, IF, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2A7B2
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells, HEK-293 cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human prostate cancer tissue, human pancreas tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
产品信息
The immunogen of 66110-1-Ig is COXIV Fusion Protein expressed in E. coli.
| Tested Applications | FC, IF, IHC, WB, ELISA |
| Cited Applications | IF, WB |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | COXIV fusion protein Ag20551 |
| Full Name | cytochrome c oxidase subunit IV isoform 1 |
| Calculated molecular weight | 19.6 kDa |
| Observed molecular weight | 17 kDa |
| GenBank accession number | BC021236 |
| Gene symbol | COX4I1 |
| Uniprot | P13073 | Gene ID (NCBI) | 1327 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COXIV antibody 66110-1-Ig | Download protocol |
| IHC protocol for COXIV antibody 66110-1-Ig | Download protocol |
| IF protocol for COXIV antibody 66110-1-Ig | Download protocol |
| FC protocol for COXIV antibody 66110-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget Prohibitin, relocated to the front ends, can control the migration directionality of colorectal cancer cells. | ||
Transl Cancer Res Hypoxia induces tumor cell growth and angiogenesis in non-small cell lung carcinoma via the Akt-PDK1-HIF1α-YKL-40 pathway. | ||
Toxicol Appl Pharmacol Berberine-photodynamic therapy sensitizes melanoma cells to cisplatin-induced apoptosis through ROS-mediated P38 MAPK pathways. | ||
Front Physiol Empagliflozin Induces White Adipocyte Browning and Modulates Mitochondrial Dynamics in KK Cg-Ay/J Mice and Mouse Adipocytes | ||
Chem Biol Interact Autophagy and mitochondrial dynamics contribute to the protective effect of diosgenin against 3-MCPD induced kidney injury. | ||
Sci Rep Jianpi Qushi Heluo Formula alleviates renal damages in Passive Hemann nephritis in rats by upregulating Parkin-mediated mitochondrial autophagy. |
