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COXIV Monoclonal antibody 5 Publications

Mouse Monoclonal| Catalog number: 66110-1-Ig |CloneNo.: 2A7B2

浓度: 1527 μg/ml

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Applications

Tested Applications:
FC, IF, IHC, WB,ELISA
Application not tested? Please see our Guarantee


Cited Applications:
IF, WB


Images See all

WB analysis using 66110-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66110-1-Ig (COXIV antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IF Staining of HepG2 using 66110-1-Ig

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66110-1-Ig (COXIV antibody, 1:100) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L); Red is the staining with alpha Tubulin antibody (11224-1-AP, 1:100).

IHC staining of human prostate cancer using 66110-1-Ig

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66110-1-Ig (COXIV antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IHC staining of human prostate cancer using 66110-1-Ig

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66110-1-Ig (COXIV antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FC experiment of HeLa using 66110-1-Ig

1X10^6 HeLa cells were stained with 0.2ug COXIV antibody (66110-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

Species Specificity:
human
Cited Species:
human, mouse
Positive WB detected in:
HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected in:
human prostate cancer tissue, human pancreas tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:2000-1:8000
IHC : 1:250-1:1000
IF : 1:50-1:500

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2a
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
COXIV fusion protein Ag20551
Full name:
cytochrome c oxidase subunit IV isoform 1
Calculated molecular weight:
19.6 kDa
Observed molecular weight:
17 kDa
GenBank accession number:
BC021236
Gene ID (NCBI):
1327
Gene symbol
COX4I1
Synonyms
COX IV 1, COX4, COX4I1, COXIV, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial

Background

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.

Protocols

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