Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 11224-1-AP

产品说明书

引用文献(1597)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (7)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

TUBA1B, Tubulin alpha 1B chain, Tubulin alpha-1B chain, Tubulin alpha, EC:3.6.5.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, rat brain
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells, MCF-7 cells, Lymphatic endothelial cells cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TUBA1B Ready-To-Use IHC Kit. TUBA1B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:3000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11224-1-AP targets Alpha Tubulin in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, rabbit, monkey, chicken, zebrafish, hamster, goat, p. lividus
免疫原 Alpha Tubulin fusion protein Ag1727 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tubulin, alpha 1b
别名 TUBA1B, Tubulin alpha 1B chain, Tubulin alpha-1B chain, Tubulin alpha, EC:3.6.5.-
计算分子量 50 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC009314
基因名称 Alpha Tubulin
Gene ID (NCBI) 10376
RRIDAB_2210206
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP68363
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the function of alpha tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.

 Where is alpha-tubulin expressed?

Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton. 

What are the post-translational modifications of alpha tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination1, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3  Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).

3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).


实验方案

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
IHC protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
IF protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
IP protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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