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GST Tag Recombinant antibody

GST Tag Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Recombinant protein

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

9B24

Cat no : 80006-1-RR

Print datasheet

Synonyms

Glutathione S-Transferase, GST, GST Tag

验证数据展示

  • WB analysis using 80006-1-RR

    WB analysis using 80006-1-RR

    GST tagged protein and other negative samples were subjected to SDS PAGE followed by western blot with 80006-1-RR (GST Tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRecombinant protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

CoIPSee 1 publications below

产品信息

80006-1-RR targets GST Tag in WB, CoIP, ELISA applications and shows reactivity with Recombinant protein samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsCoIP
Tested Reactivity Recombinant protein
Immunogen GST Tag fusion protein Ag0040 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name GST Tag
Synonyms Glutathione S-Transferase, GST, GST Tag
Calculated Molecular Weight 26 kDa
GenBank Accession NumberFN315687
Gene Symbol
Gene ID (NCBI)
RRIDAB_2882936
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody is a Rabbit recombinant antibody to GST tag.

实验方案

Product Specific Protocols
WB protocol for GST Tag antibody 80006-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
CoIP

Vet Microbiol

PEDV N protein capture protein translation element PABPC1 and eIF4F to promote viral replication

Authors - Huanjie Zhai
View Article