|Positive WB detected in||HEK-293 cells, pig liver tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A431 cells, HeLa cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:325-1:1300|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66166-1-Ig targets TBP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Applications||FC, IF, IHC, IP, WB, ELISA|
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||TBP fusion protein Ag12383|
|Full Name||TATA box binding protein|
|Calculated molecular weight||338 aa, 38 kDa|
|Observed molecular weight||mouse/rat 33-36 kDa and human 37-43kDa|
|GenBank accession number||BC110341|
|Uniprot||P20226||Gene ID (NCBI)||6908|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).
|Product Specific Protocols|
|WB protocol for TBP antibody 66166-1-Ig||Download protocol|
|IHC protocol for TBP antibody 66166-1-Ig||Download protocol|
|IF protocol for TBP antibody 66166-1-Ig||Download protocol|
|IP protocol for TBP antibody 66166-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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