• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66166-1-Ig (same clone as 66166-1-PBS)

    WB analysis using 66166-1-Ig (same clone as 66166-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 66166-1-Ig (same clone as 66166-1-PBS)

    IP experiment of HEK-293 using 66166-1-Ig (same clone as 66166-1-PBS)

    IP result of anti-TBP (IP:66166-1-Ig, 3ug; Detection:66166-1-Ig 1:800) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66166-1-Ig (same clone as 66166-1-PBS)

    IHC staining of human liver cancer using 66166-1-Ig (same clone as 66166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:650 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66166-1-Ig (same clone as 66166-1-PBS)

    IF Staining of HeLa using 66166-1-Ig (same clone as 66166-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66166-1-Ig(TBP antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • WB analysis using 66166-1-Ig (same clone as 66166-1-PBS)

    WB analysis using 66166-1-Ig (same clone as 66166-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66166-1-Ig (same clone as 66166-1-PBS)

    WB analysis of HEK-293 using 66166-1-Ig (same clone as 66166-1-PBS)

    Western blot analysis of TBP in various cell lines using Proteintech antibody 66166-1-Ig at a dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66166-1-Ig (same clone as 66166-1-PBS)

    IF Staining of A431 using 66166-1-Ig (same clone as 66166-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TBP antibody (66166-1-Ig, Clone: 2H3B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

TBP Monoclonal antibody, PBS Only

TBP Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66166-1-PBS

产品说明书

CloneNo. 2H3B2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

TATA sequence binding protein, TATA box factor, TATA box binding protein, TATA binding factor, GTF2D1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

66166-1-PBS targets TBP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 TBP fusion protein Ag12383 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 TATA box binding protein
别名 TATA sequence binding protein, TATA box factor, TATA box binding protein, TATA binding factor, GTF2D1
计算分子量 338 aa, 38 kDa
观测分子量 mouse/rat 33-36 kDa and human 37-43kDa
GenBank蛋白编号BC110341
基因名称 TBP
Gene ID (NCBI) 6908
RRIDAB_2881562
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20226
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).