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TBP Monoclonal antibody, PBS Only

TBP Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H3B2

Cat no : 66166-1-PBS

Print datasheet

Synonyms

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID

验证数据展示

  • WB analysis using 66166-1-Ig (same clone as 66166-1-PBS)

    WB analysis using 66166-1-Ig (same clone as 66166-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 66166-1-Ig (same clone as 66166-1-PBS)

    IP experiment of HEK-293 using 66166-1-Ig (same clone as 66166-1-PBS)

    IP result of anti-TBP (IP:66166-1-Ig, 3ug; Detection:66166-1-Ig 1:800) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66166-1-Ig (same clone as 66166-1-PBS)

    IHC staining of human liver cancer using 66166-1-Ig (same clone as 66166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:650 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66166-1-Ig (same clone as 66166-1-PBS)

    IF Staining of HeLa using 66166-1-Ig (same clone as 66166-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66166-1-Ig(TBP antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • WB analysis using 66166-1-Ig (same clone as 66166-1-PBS)

    WB analysis using 66166-1-Ig (same clone as 66166-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66166-1-Ig (same clone as 66166-1-PBS)

    WB analysis of HEK-293 using 66166-1-Ig (same clone as 66166-1-PBS)

    Western blot analysis of TBP in various cell lines using Proteintech antibody 66166-1-Ig at a dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66166-1-Ig (same clone as 66166-1-PBS)

    IF Staining of A431 using 66166-1-Ig (same clone as 66166-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TBP antibody (66166-1-Ig, Clone: 2H3B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66166-1-PBS in a different storage buffer formulation.

产品信息

66166-1-PBS targets TBP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen TBP fusion protein Ag12383 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name TATA box binding protein
Synonyms GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID
Calculated Molecular Weight 338 aa, 38 kDa
Observed Molecular Weight mouse/rat 33-36 kDa and human 37-43kDa
GenBank Accession NumberBC110341
Gene Symbol TBP
Gene ID (NCBI) 6908
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20226
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).