CoraLite®555-conjugated TBP Monoclonal antibody
TBP Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
2H3B2
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
| Positive FC (Intra) detected in | HeLa cells, HeLa cell line |
For other applications, we recommend the unconjugated version of this antibody, 66166-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-66166 targets TBP in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat, pig |
| Immunogen | TBP fusion protein Ag12383 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | TATA box binding protein |
| Synonyms | TATA sequence binding protein, TATA box factor, TATA box binding protein, TATA binding factor, GTF2D1 |
| Calculated Molecular Weight | 338 aa, 38 kDa |
| Observed Molecular Weight | mouse/rat 33-36 kDa and human 37-43kDa |
| GenBank Accession Number | BC110341 |
| Gene Symbol | TBP |
| Gene ID (NCBI) | 6908 |
| RRID | AB_2919676 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 557 nm / 570 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P20226 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 TBP antibody CL555-66166 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
