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VDAC1/Porin Monoclonal antibody

VDAC1/Porin Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF-P, FC (Intra), ELISA and More (4)

Conjugate

Unconjugated

CloneNo.

1E2C7

Cat no : 66345-1-Ig

Print datasheet

Synonyms

Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin

验证数据展示

  • WB analysis using 66345-1-Ig

    WB analysis using 66345-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of mouse liver using 66345-1-Ig

    IF Staining of mouse liver using 66345-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using VDAC1/Porin antibody (66345-1-Ig, Clone: 1E2C7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 66345-1-Ig

    FC experiment of HepG2 using 66345-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug VDAC1/Porin Monoclonal antibody (66345-1-Ig, Clone:1E2C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG3 isotype control Mouse McAb (66360-4-Ig, Clone: 1H4A5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inMDA-MB-231 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, RAW264.7 cells
Positive IF-P detected inmouse liver tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VDAC1 Ready-To-Use IHC Kit. VDAC1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 750 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66345-1-Ig targets VDAC1/Porin in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name voltage-dependent anion channel 1
Synonyms Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 35-37 kDa
GenBank Accession NumberNM_003374
Gene Symbol Porin
Gene ID (NCBI) 7416
RRIDAB_2881725
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21796
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904).

实验方案

Product Specific Protocols
WB protocol for VDAC1/Porin antibody 66345-1-IgDownload protocol
IF protocol for VDAC1/Porin antibody 66345-1-IgDownload protocol
FC protocol for VDAC1/Porin antibody 66345-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma

Authors - Qifan Hu
View Article
WB

Proc Natl Acad Sci U S A

Ferroptosis as a target for protection against cardiomyopathy.

Authors - Xuexian Fang
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humanWB

Free Radic Biol Med

The interaction of Atg4B and Bcl-2 plays an important role in Cd-induced crosstalk between apoptosis and autophagy through disassociation of Bcl-2-Beclin1 in A549 cells.

Authors - Zhiguo Li
View Article
mouse

Diabetes

PACS-2 Ameliorates Tubular Injury by Facilitating Endoplasmic Reticulum-Mitochondria Contact and Mitophagy in Diabetic Nephropathy.

Authors - Chenrui Li
View Article
mouseWB

Oxid Med Cell Longev

The lncRNA-AK046375 Upregulates Metallothionein-2 by Sequestering miR-491-5p to Relieve the Brain Oxidative Stress Burden after Traumatic Brain Injury.

Authors - Wei Tang
View Article
mouseWB

Cell Death Discov

Idebenone improves motor dysfunction, learning and memory by regulating mitophagy in MPTP-treated mice.

Authors - Junqiang Yan
View Article