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CoraLite® Plus 488-conjugated VDAC1/Porin Monoclonal antibody

VDAC1/Porin Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E2C7

Cat no : CL488-66345

Print datasheet

Synonyms

Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin

验证数据展示

  • FC experiment of HepG2 using CL488-66345

    FC experiment of HepG2 using CL488-66345

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Vdac1/Porin Monoclonal Antibody (CL488-66345, Clone:1E2C7)(red), or 0.8 ug CoraLite® Plus 488-conjugated Mouse IgG3 isotype control Mouse McAb (CL488-66360-4, Clone: 1H4A5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VDAC1 Ready-To-Use IHC Kit. VDAC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66345-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66345 targets VDAC1/Porin in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name voltage-dependent anion channel 1
Synonyms Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 35-37 kDa
GenBank Accession NumberNM_003374
Gene Symbol Porin
Gene ID (NCBI) 7416
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21796
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 VDAC1/Porin antibody CL488-66345Download protocol
Standard Protocols
Click here to view our Standard Protocols