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Conjugate
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验证数据展示

  • WB analysis using 66345-1-Ig (same clone as 66345-1-PBS)

    WB analysis using 66345-1-Ig (same clone as 66345-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66345-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 66345-1-Ig (same clone as 66345-1-PBS)

    IF Staining of mouse liver using 66345-1-Ig (same clone as 66345-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using VDAC1/Porin antibody (66345-1-Ig, Clone: 1E2C7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66345-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66345-1-Ig (same clone as 66345-1-PBS)

    FC experiment of HepG2 using 66345-1-Ig (same clone as 66345-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug VDAC1/Porin Monoclonal antibody (66345-1-Ig, Clone:1E2C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG3 isotype control Mouse McAb (66360-4-Ig, Clone: 1H4A5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66345-1-PBS in a different storage buffer formulation.

VDAC1/Porin Monoclonal antibody, PBS Only

VDAC1/Porin Monoclonal Antibody for WB, IF-P, FC (Intra), Indirect ELISA
Cat No. 66345-1-PBS

产品说明书

CloneNo. 1E2C7

宿主/亚型

Mouse / IgG3

种属反应性

human, mouse, rat

应用

WB, IF-P, FC (Intra), Indirect ELISA

别名

Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy VDAC1 Ready-To-Use IHC Kit. VDAC1 primary antibody included.

产品信息

66345-1-PBS targets VDAC1/Porin in WB, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 voltage-dependent anion channel 1
别名 Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin
计算分子量 31 kDa
观测分子量 35-37 kDa
GenBank蛋白编号NM_003374
基因名称 Porin
Gene ID (NCBI) 7416
RRIDAB_2881725
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21796
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904).