VDAC1/Porin Monoclonal antibody
VDAC1/Porin Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG3
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF-P, FC (Intra), ELISA and More (3)
Conjugate
Unconjugated
CloneNo.
1E2C7
验证数据展示
经过测试的应用
| Positive WB detected in | MDA-MB-231 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, RAW264.7 cells |
| Positive IHC detected in | human heart tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy VDAC1 Ready-To-Use IHC Kit. VDAC1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 750 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66345-1-Ig targets VDAC1/Porin in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | voltage-dependent anion channel 1 |
| Synonyms | Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 35-37 kDa |
| GenBank Accession Number | NM_003374 |
| Gene Symbol | Porin |
| Gene ID (NCBI) | 7416 |
| RRID | AB_2881725 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P21796 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VDAC1/Porin antibody 66345-1-Ig | Download protocol |
| IHC protocol for VDAC1/Porin antibody 66345-1-Ig | Download protocol |
| IF protocol for VDAC1/Porin antibody 66345-1-Ig | Download protocol |
| FC protocol for VDAC1/Porin antibody 66345-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med The interaction of Atg4B and Bcl-2 plays an important role in Cd-induced crosstalk between apoptosis and autophagy through disassociation of Bcl-2-Beclin1 in A549 cells. | ||
Diabetes PACS-2 Ameliorates Tubular Injury by Facilitating Endoplasmic Reticulum-Mitochondria Contact and Mitophagy in Diabetic Nephropathy. | ||
Oxid Med Cell Longev The lncRNA-AK046375 Upregulates Metallothionein-2 by Sequestering miR-491-5p to Relieve the Brain Oxidative Stress Burden after Traumatic Brain Injury. | ||
Cell Death Discov Idebenone improves motor dysfunction, learning and memory by regulating mitophagy in MPTP-treated mice. | ||
Int J Mol Sci PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway. |
