验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue, NIH/3T3 cells, HSC-T6 cells, K-562 cells, Jurakat cells, HEK-293 cells, HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 11 publications below |
产品信息
81640-5-RR targets GAPDH in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | GAPDH fusion protein Ag0766 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Synonyms | 1H18 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC004109 |
| Gene Symbol | GAPDH |
| Gene ID (NCBI) | 2597 |
| RRID | AB_3086559 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04406 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GAPDH antibody 81640-5-RR | Download protocol |
| IF protocol for GAPDH antibody 81640-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Sci FKBP10 Promotes the Muscle Invasion of Bladder Cancer via Lamin A Dysregulation | ||
Respirology Allele-specific micro-RNA-mediated regulation of ADAM33 in childhood allergic asthma | ||
Open Med (Wars) Comprehensive landscape of integrator complex subunits and their association with prognosis and tumor microenvironment in gastric cancer | ||
J Neuroinflammation Exercise training upregulates CD55 to suppress complement-mediated synaptic phagocytosis in Parkinson's disease | ||
Ecotoxicol Environ Saf The role of Drp1 - Pink1 - Parkin - mediated mitophagy in perfluorobutane sulfonate- induced hepatocyte damage | ||
Eur J Pharmacol Tumoral EIF4EBP1 regulates the crosstalk between tumor-associated macrophages and tumor cells in MRTK |