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Beta Tubulin Monoclonal antibody 113 Publications

Mouse Monoclonal| Catalog number: 66240-1-Ig |CloneNo.: 1D4A4

浓度: 1427 μg/ml

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Applications

Tested Applications:
IF, IHC, IP, WB,ELISA
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Cited Applications:
IF, WB


Images See all

WB analysis using 66240-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66240-1-Ig (beta Tubulin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

WB analysis using 66240-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66240-1-Ig (beta Tubulin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

IF Staining of HeLa using 66240-1-Ig

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66240-1-Ig (beta Tubulin antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: staning with CoraLite555-Phalloidin.

IHC staining of human brain using 66240-1-Ig

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 10x lens).

IHC staining of human brain using 66240-1-Ig

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 40x lens).

IP experiment of HeLa using 66240-1-Ig

IP Result of anti-beta Tubulin (IP:66240-1-Ig, 5ug; Detection:66240-1-Ig 1:1000) with HeLa cells lysate 880ug.

Species Specificity:
human, mouse, rat, nematode, pig, zebrafish
Cited Species:
human, mouse, pig, rabbit, rat
Positive WB detected in:
HeLa cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human brain tissue, human breast cancer tissue, rat brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells, HepG2 cells
Recommended dilution:
WB : 1:20000-1:100000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:1000
IF : 1:200-1:800

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2a
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Beta Tubulin fusion protein Ag0117
Full name:
tubulin, beta 3
Calculated molecular weight:
450 aa, 50 kDa
Observed molecular weight:
50-55 kDa
GenBank accession number:
BC000748
Gene ID (NCBI):
10381
Gene symbol
TUBB3
Synonyms
beta tubulin, MC1R, TUBB, Tubulin-beta

Background

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha and beta tubulins have been widely used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

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