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验证数据展示

  • WB analysis using 66240-1-Ig (same clone as 66240-1-PBS)

    WB analysis using 66240-1-Ig (same clone as 66240-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66240-1-Ig (beta Tubulin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

  • WB analysis using 66240-1-Ig (same clone as 66240-1-PBS)

    WB analysis using 66240-1-Ig (same clone as 66240-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66240-1-Ig (beta Tubulin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

    IF Staining of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66240-1-Ig (beta Tubulin antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: staning with CoraLite555-Phalloidin. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 66240-1-Ig (same clone as 66240-1-PBS)

    IHC staining of human brain using 66240-1-Ig (same clone as 66240-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IHC staining of human brain using 66240-1-Ig (same clone as 66240-1-PBS)

    IHC staining of human brain using 66240-1-Ig (same clone as 66240-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IP experiment of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

    IP experiment of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

    IP result of anti-Beta Tubulin (IP:66240-1-Ig, 5ug; Detection:66240-1-Ig 1:1000) with HeLa cells lysate 880ug. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse eye using 66240-1-Ig (same clone as 66240-1-PBS)

    IF Staining of mouse eye using 66240-1-Ig (same clone as 66240-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Beta Tubulin antibody (66240-1-Ig, Clone: 1D4A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

    IF Staining of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (66240-1-Ig, Clone: 1D4A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

Beta Tubulin Monoclonal antibody, PBS Only

Beta Tubulin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 66240-1-PBS

产品说明书

CloneNo. 1D4A4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, zebrafish, nematode

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA

别名

Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.

产品信息

66240-1-PBS targets Beta Tubulin in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, zebrafish, nematode samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
经测试反应性 human, mouse, rat, pig, zebrafish, nematode
免疫原 Beta Tubulin fusion protein Ag0117 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 tubulin, beta 3
别名 Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4
计算分子量 450 aa, 50 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC000748
基因名称 TUBB3
Gene ID (NCBI) 10381
RRIDAB_2881629
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13509
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The alpha and beta tubulins (+/- 55 kDa MW) are homologous but are not identical. Beta tubulins have been widely used as loading control.

 

What is the molecular weight of beta-tubulin? Are there any isoforms of beta-tubulin?

The molecular weight of tubulin is 50-52 kDa. Humans have eight beta-tubulin isotypes, encoded by different genes, that differ in their C-terminal sequences. They have different tissue expression profiles and can rise to microtubules of different properties (PMID: 20191564).

 

How to use beta-tubulin as a loading control

Beta-tubulin is one of the most commonly used references as a loading control for cell lysates in western blotting. It is abundantly expressed across various tissues and developmental stages and highly conserved across species. However, since some variability has been observed in the expression levels of commonly used housekeeping genes (PMID: 15627964), it is recommended that more than one loading control antibody is used while developing new assays. More information can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.

 

What drugs can influence beta-tubulin and organization of microtubules?

Many drugs that affect microtubule dynamics target beta-tubulin, mainly by interfering with the GTP hydrolysis (PMID: 21381049). Paclitaxel (Taxol) is used to stabilize microtubules by slowing down their depolymerization, while colchicine and vinca alkaloids (vinblastine) destabilize microtubules. They are used in research and also in the clinic as anti-cancer agents.

 

Is beta-tubulin post-translationally modified?

Yes, tubulins are subject to extensive post-translational modifications (PTMs) that affect the organization of microtubules and their dynamics. The most common modifications include polyglutamylation, polyglycylation, polyamination, glycososylation, glycation, phosphorylation, and acetylation (PMID: 24801181 and 25468068).