CoraLite® Plus 647-conjugated MYC tag Monoclonal antibody

MYC tag Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-60003

产品说明书

CloneNo. 1A5A2

宿主/亚型

Mouse / IgG1

种属反应性

recombinant protein

应用

IF/ICC, FC (Intra)

EQKLISEEDL

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inTransfected HEK-293 cells
Positive FC (Intra) detected inTransfected HEK-293 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-60003 targets MYC tag in IF/ICC, FC (Intra) applications and shows reactivity with recombinant protein samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 recombinant protein
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Myc tag
别名 EQKLISEEDL
基因名称 Myc tag
Gene ID (NCBI) 99
RRIDAB_2920241
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein G purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 MYC tag antibody CL647-60003Download protocol
FC protocol for CL Plus 647 MYC tag antibody CL647-60003Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Biochem Pharmacol

Peptide-based CAR-NK cells: A novel strategy for the treatment of solid tumors

Authors - Qianqian Wang
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