CoraLite® Plus 750-conjugated MYC tag Monoclonal antibody
MYC tag Monoclonal Antibody for WB, IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
recombinant protein
Applications
WB, IF/ICC
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
1A5A2
验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-60003 targets MYC tag in WB, IF/ICC applications and shows reactivity with recombinant protein samples.
| Tested Applications | WB, IF/ICC Application Description |
| Tested Reactivity | recombinant protein |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | MYC tag |
| Synonyms | myc, EQKLISEEDL |
| Gene Symbol | |
| Gene ID (NCBI) | |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 MYC tag antibody CL750-60003 | Download protocol |
| IF protocol for CL Plus 750 MYC tag antibody CL750-60003 | Download protocol |
| FC protocol for CL Plus 750 MYC tag antibody CL750-60003 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |