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验证数据展示

  • IF Staining of Transfected HEK-293 using CL594-60003

    IF Staining of Transfected HEK-293 using CL594-60003

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite®594 MYC tag antibody (CL594-60003, Clone: 1A5A2 ) at dilution of 1:200.

  • FC experiment of HEK-293 transfected with GFP-MYC using CL594-60003

    FC experiment of HEK-293 transfected with GFP-MYC using CL594-60003

    1x10^6 HEK-293 transfected with GFP-MYC were intracellularly stained with 0.4 ug CoraLite®594-conjugated MYC tag Monoclonal antibody (CL594-60003, Clone:1A5A2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated MYC tag Monoclonal antibody

MYC tag Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-60003
CloneNo. 1A5A2

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

IF/ICC, FC (Intra)

Synonyms

myc, EQKLISEEDL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inTransfected HEK-293 cells
Positive FC (Intra) detected inTransfected HEK-293 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60003 targets MYC tag in IF/ICC, FC (Intra) applications and shows reactivity with recombinant protein samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity recombinant protein
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name MYC tag
Synonyms myc, EQKLISEEDL
Gene Symbol
Gene ID (NCBI)
RRIDAB_3673432
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

实验方案

Product Specific Protocols
IF protocol for CL594 MYC tag antibody CL594-60003Download protocol
FC protocol for CL594 MYC tag antibody CL594-60003Download protocol
Standard Protocols
Click here to view our Standard Protocols