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HRP-conjugated MYC tag Monoclonal antibody

MYC tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB

Conjugate

HRP

CloneNo.

1A5A2

Cat No : HRP-60003

Print datasheet

Synonyms

EQKLISEEDL, myc, Myc tag, MYC-tag

验证数据展示

  • WB analysis using HRP-60003

    WB analysis using HRP-60003

    Various lysates were subjected to SDS PAGE followed by western blot with HRP-60003 (MYC tag antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293T cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

HRP-60003 targets MYC tag in WB applications and shows reactivity with recombinant protein samples.

Tested Applications WB Application Description
Tested Reactivity recombinant protein
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name MYC tag
Synonyms EQKLISEEDL, myc, Myc tag, MYC-tag
Gene Symbol
Gene ID (NCBI)
Conjugate HRP
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

实验方案

Product Specific Protocols
WB protocol for HRP MYC tag antibody HRP-60003Download protocol
Standard Protocols
Click here to view our Standard Protocols