MYC tag Monoclonal antibody
MYC tag Monoclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
recombinant protein and More (2)
Applications
IF, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1A5A2
验证数据展示
经过测试的应用
| Positive WB detected in | recombinant protein |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF detected in | Transfected HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
产品信息
60003-2-Ig targets MYC tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Applications | IF, IP, WB, ELISA |
| Cited Applications | CoIP, IF, IP, WB |
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | MYC tag |
| Gene symbol | |
| Gene ID (NCBI) | |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MYC tag antibody 60003-2-Ig | Download protocol |
| IP protocol for MYC tag antibody 60003-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Mol Med The pattern-recognition molecule mindin binds integrin Mac-1 to promote macrophage phagocytosis via Syk activation and NF-κB p65 translocation. | ||
Elife Distinct RanBP1 nuclear export and cargo dissociation mechanisms between fungi and animals. | ||
Proc Natl Acad Sci U S A SENCR stabilizes vascular endothelial cell adherens junctions through interaction with CKAP4. | ||
Commun Biol Phosphoproteomics identifies dual-site phosphorylation in an extended basophilic motif regulating FILIP1-mediated degradation of filamin-C. | ||
Mol Plant Pathol Pm21 CC domain activity modulated by intramolecular interactions is implicated in cell death and disease resistance. | ||
Biomolecules (20S)G-Rh2 Inhibits NF-κB Regulated Epithelial-Mesenchymal Transition by Targeting Annexin A2. |
