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MYC tag Monoclonal antibody, PBS Only

MYC tag Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

1A5A2

Cat no : 60003-2-PBS

Print datasheet

Synonyms

myc, EQKLISEEDL

验证数据展示

  • WB analysis of recombinant proein using 60003-2-Ig (same clone as 60003-2-PBS)

    WB analysis of recombinant proein using 60003-2-Ig (same clone as 60003-2-PBS)

    Western blot of Myc-tagged fusion protein with anti-Myc-tag (60003-2-Ig) at various dilutions. This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

  • IF Staining of Transfected HEK-293 using 60003-2-Ig (same clone as 60003-2-PBS)

    IF Staining of Transfected HEK-293 using 60003-2-Ig (same clone as 60003-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using MYC tag antibody (60003-2-Ig, Clone: 1A5A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

  • IP experiment of Transfected HEK-293 using 60003-2-Ig (same clone as 60003-2-PBS)

    IP experiment of Transfected HEK-293 using 60003-2-Ig (same clone as 60003-2-PBS)

    IP result of anti-MYC tag (IP:60003-2-Ig, 4ug; Detection:60003-2-Ig 1:10000) with Transfected HEK-293 cells lysate 300ug. This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 transfected with GFP-MYC using 60003-2-Ig (same clone as 60003-2-PBS)

    FC experiment of HEK-293 transfected with GFP-MYC using 60003-2-Ig (same clone as 60003-2-PBS)

    1x10^6 HEK-293 transfected with GFP-MYC were intracellularly stained with 0.25 ug MYC tag Monoclonal antibody (60003-2-Ig, Clone:1A5A2) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

产品信息

60003-2-PBS targets MYC tag in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications WB, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity recombinant protein
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name MYC tag
Synonyms myc, EQKLISEEDL
Gene Symbol
Gene ID (NCBI)
RRIDAB_2734122
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.