验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue, human liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ALB Ready-To-Use IHC Kit. ALB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 750 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66051-1-Ig targets Albumin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, rat, pig |
| 文献引用反应性 | human, mouse, rat, pig, rabbit |
| 免疫原 | Albumin fusion protein Ag9885 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | albumin |
| 别名 | ALB, Serum albumin, HSA, 4A1C11 |
| 计算分子量 | 609 aa, 69 kDa |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | BC034023 |
| 基因名称 | Albumin |
| Gene ID (NCBI) | 213 |
| RRID | AB_11042320 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P02768 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Albumin antibody 66051-1-Ig | Download protocol |
| IHC protocol for Albumin antibody 66051-1-Ig | Download protocol |
| IF protocol for Albumin antibody 66051-1-Ig | Download protocol |
| IP protocol for Albumin antibody 66051-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans. | ||
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
J Extracell Vesicles Optimized AF4 combined with density cushion ultracentrifugation enables profiling of high-purity human blood extracellular vesicles | ||
J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. |
