Albumin Monoclonal antibody
Albumin Monoclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, pig and More (2)
Applications
IF, IHC, IP, WB,ELISA
Conjugate
Unconjugated
CloneNo.
4A1C11
验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue, human liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
产品信息
The immunogen of 66051-1-Ig is Albumin Fusion Protein expressed in E. coli.
| Tested Applications | IF, IHC, IP, WB,ELISA |
| Cited Applications | IF, IP, WB |
| Tested Reactivity | human, rat, pig |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Albumin fusion protein Ag9885 |
| Full Name | albumin |
| Calculated molecular weight | 609 aa, 69 kDa |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC034023 |
| Gene symbol | ALB |
| Uniprot | P02768 | Gene ID (NCBI) | 213 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Albumin antibody 66051-1-Ig | Download protocol |
| IHC protocol for Albumin antibody 66051-1-Ig | Download protocol |
| IF protocol for Albumin antibody 66051-1-Ig | Download protocol |
| IP protocol for Albumin antibody 66051-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Neurosci Methods Total Protein is an Effective Loading Control for Cerebrospinal Fluid Western Blots. | ||
Cell Death Dis WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1. | ||
Nanotechnology Kupffer Cell-targeting strategy for the protection of Hepatic Ischemia/Reperfusion Injury. | ||
Life Sci Continuous zebularine treatment enhances hepatic differentiation of mesenchymal stem cells under liver-specific factors induction in vitro. | ||
Biochim Biophys Acta Mol Basis Dis The Annexin A2-Notch regulatory loop in hepatocytes promotes liver fibrosis in NAFLD by increasing osteopontin expression. | ||
Stem Cell Res Ther Treatment with adipose tissue-derived mesenchymal stem cells exerts anti-diabetic effects, improves long-term complications, and attenuates inflammation in type 2 diabetic rats. |
