Albumin Monoclonal antibody

Albumin Monoclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig and More (2)

Applications

IF, IHC, IP, WB,ELISA

Conjugate

Unconjugated

CloneNo.

4A1C11

Cat No : 66051-1-Ig

Print datasheet

Synonyms

Alb, albumin, Serum albumin



经过测试的应用

Positive WB detected inhuman plasma tissue, human liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

产品信息

The immunogen of 66051-1-Ig is Albumin Fusion Protein expressed in E. coli.

Tested Applications IF, IHC, IP, WB,ELISA
Cited Applications IF, IP, WB
Tested Reactivity human, rat, pig
Cited Reactivityhuman, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Albumin fusion protein Ag9885
Full Name albumin
Calculated molecular weight 609 aa, 69 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC034023
Gene symbol ALB
UniprotP02768
Gene ID (NCBI) 213
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.

实验方案

Product Specific Protocols
WB protocol for Albumin antibody 66051-1-IgDownload protocol
IHC protocol for Albumin antibody 66051-1-IgDownload protocol
IF protocol for Albumin antibody 66051-1-IgDownload protocol
IP protocol for Albumin antibody 66051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Neurosci Methods

Total Protein is an Effective Loading Control for Cerebrospinal Fluid Western Blots.

Authors - Mahlon A Collins
humanIF

Cell Death Dis

WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1.

Authors - Zhe Zheng
mouseIF

Nanotechnology

Kupffer Cell-targeting strategy for the protection of Hepatic Ischemia/Reperfusion Injury.

Authors - Qingqing Dai
rabbitWB

Life Sci

Continuous zebularine treatment enhances hepatic differentiation of mesenchymal stem cells under liver-specific factors induction in vitro.

Authors - Juan Chen
human,mouseIF

Biochim Biophys Acta Mol Basis Dis

The Annexin A2-Notch regulatory loop in hepatocytes promotes liver fibrosis in NAFLD by increasing osteopontin expression.

Authors - Guangyan Wang
ratIF

Stem Cell Res Ther

Treatment with adipose tissue-derived mesenchymal stem cells exerts anti-diabetic effects, improves long-term complications, and attenuates inflammation in type 2 diabetic rats.

Authors - Songyan Yu