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验证数据展示

  • IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

    IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALB antibody included
  • IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

    IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALB antibody included
  • WB analysis of human plasma using 66051-1-Ig (same clone as 66051-1-PBS)

    WB analysis of human plasma using 66051-1-Ig (same clone as 66051-1-PBS)

    human plasma (diluted 5000 fold) was subjected to SDS PAGE followed by western blot with 66051-1-Ig (Albumin Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50031-1

    Sandwich ELISA standard curve of MP50031-1

    Sandwich ELISA standard curve of MP50031-1, Albumin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66051-2-PBS. Detection antibody: 66051-1-PBS. Standard: Ag9885. Range: 50-3200 ng/mL.

  • Sample test of MP50031-1

    Sample test of MP50031-1

    Serum of four individual healthy human donors was measured. The Albumin concentration of detected samples was determined to be 29.65 mg/mL with a range of 11.40-48.00 mg/mL.

  • Sample test of MP50031-1

    Sample test of MP50031-1

    Urine of four individual healthy human donors was measured. The Albumin concentration of detected samples was determined to be 1,036.00 ng/mL with a range of 560.00-1,616.00 ng/mL.

  • Sample test of MP50031-1

    Sample test of MP50031-1

    Saliva of two individual healthy human donors was measured. The Albumin concentrations of detected samples were determined to be 32.00 μg/mL and 18.60 μg/mL, respectively.

  • Sample test of MP50031-1

    Sample test of MP50031-1

    Human milk of two individual healthy human donors was measured. The Albumin concentrations of detected samples were determined to be 106.00 μg/mL and 116.00 μg/mL, respectively.

  • Sample test of MP50031-1

    Sample test of MP50031-1

    Human cerebrospinal fluid (CSF) of one individual human donors was measured. The Albumin concentration of detected samples was determined to be 29.50 μg/mL.

  • IP experiment of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

    IP experiment of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

    IP result of anti-Albumin (IP:66051-1-Ig, 4ug; Detection:66051-1-Ig 1:1000) with HepG2 cells lysate 1720 ug. This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

    IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66051-1-Ig (Albumin antibody) at dilution of 1:64000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALB antibody included
  • IF Staining of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

    IF Staining of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

    IF Staining of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 66051-1-Ig

    Affinity and Kinetic Characterization of 66051-1-Ig

    Biolayer interferometry (BLl) kinetic assays of 66051-1-Ig against Human Albumin were performed. The affinity constant is 0.574 nM

Albumin Monoclonal antibody, PBS Only (Detector)

Albumin Monoclonal Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 66051-1-PBS

产品说明书

CloneNo. 4A1C11

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig

应用

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

别名

ALB, 4A1C11, HSA, Serum albumin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ALB Ready-To-Use IHC Kit. ALB primary antibody included.

产品信息

66051-1-PBS targets Albumin as part of a matched antibody pair:

MP50031-1: 66051-2-PBS capture and 66051-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, rat, pig
免疫原 Albumin fusion protein Ag9885 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 albumin
别名 ALB, 4A1C11, HSA, Serum albumin
计算分子量 609 aa, 69 kDa
观测分子量 66 kDa
GenBank蛋白编号BC034023
基因名称 Albumin
Gene ID (NCBI) 213
RRIDAB_11042320
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02768
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.