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PCNA Polyclonal antibody

PCNA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (8)





Cat No : 10205-2-AP

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Positive WB detected in4T1 cells, A431 cells, NIH/3T3 cells, HepG2 cells, mouse testis tissue, HeLa cells, Raji cells, Jurkat cells, mouse liver tissue, HEK-293 cells, rat liver tissue, rat testis tissue, HEK293 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman stomach cancer tissue, mouse liver tissue, human colon cancer tissue, human liver cancer tissue, human malignant melanoma tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Neuro-2a cells, MCF-7 cells, human breast cancer tissue
Positive FC detected inJurkat cells


Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery


10205-2-AP targets PCNA in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, Alligator, bovine, chicken, ducks, fish, medaka embryos, pig, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCNA fusion protein Ag0277
Full Name proliferating cell nuclear antigen
Calculated molecular weight 29 kDa/31 kDa
Observed molecular weight 36-38 kDa
GenBank accession numberBC000491
Gene symbol PCNA
Gene ID (NCBI) 5111
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).


Product Specific Protocols
WB protocol for PCNA antibody 10205-2-APDownload protocol
IHC protocol for PCNA antibody 10205-2-APDownload protocol
IF protocol for PCNA antibody 10205-2-APDownload protocol
IP protocol for PCNA antibody 10205-2-APDownload protocol
Standard Protocols
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