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Transferrin Polyclonal antibody 21 Publications

Rabbit Polyclonal| Catalog number: 17435-1-AP

Featured Product KD/KO validated

浓度: 500 μg/ml

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Applications

Tested Applications:
IF, IHC, WB,ELISA
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Cited Applications:
IF, IHC, WB


Images See all

WB analysis using 17435-1-AP

human urine lysates were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IHC staining of human hepatocirrhosis using 17435-1-AP

Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 10x lens).

IHC staining of human hepatocirrhosis using 17435-1-AP

Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 40x lens).

IF Staining of HepG2 using 17435-1-AP

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 17435-1-AP (Transferrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Species Specificity:
human, mouse, rat
Cited Species:
human, mouse, rat
Positive WB detected in:
human urine sample, mouse kidney tissue, mouse spleen tissue, rat kidney tissue
Positive IHC detected in:
human hepatocirrhosis tissue, human placenta tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:1000-1:5000
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Transferrin fusion protein Ag11504
Full name:
transferrin
Calculated molecular weight:
698 aa, 77 kDa
Observed molecular weight:
77 kDa
GenBank accession number:
BC059367
Gene ID (NCBI):
7018
Gene symbol
TF
Synonyms
Beta 1 metal binding globulin, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, Transferrin

Background

Serotransferrin(TF) is a 77 kDa secreted protein which is also named as transferrin, siderophilin, beta-1 metal-binding globulin and belongs to a family of homologous iron-binding glycoproteins that encompasses lactoferrin (found both intracellular and in secretions, including milk), melanotrasferrin (present on melanoma cells) and ovotransferrin (present in egg white). TF is a multi-function protein with a primary role in transporting iron in a safe, redox-inactive state from absorption to utilization or storage sites around the body. The association of Tf with the immune system derives from its ability to restrict serum free-iron levels, creating low-iron environments where the infection capacity of pathogenic microorganisms is limited. TF is expressed predominantly in the liver and secreted in plasma, but lower amounts can be synthesized in other tissues such as the brain and the testis

Protocols

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