ATP1A1 Recombinant antibody

ATP1A1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83191-6-RR

产品说明书

CloneNo. 240160E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1, ATP1A 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 647
规格: 

-/ -


经过测试的应用

Positive WB detected inunboiled A431 cells, unboiled HeLa cells, unboiled RAW 264.7 cells
Positive IF/ICC detected inA431 cells, HEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

For optimal WB detection with 83191-6-RR, we do not recommend boiling the sample after lysis.

Planning an IHC experiment? We recommend our IHCeasy ATP1A1 Ready-To-Use IHC Kit. ATP1A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83191-6-RR targets ATP1A1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 ATP1A1 fusion protein Ag5763 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, Na+/K+ transporting, alpha 1 polypeptide
别名 Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1, ATP1A 1
计算分子量 113 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC050359
基因名称 ATP1A1
Gene ID (NCBI) 476
ENSEMBL Gene IDENSG00000163399
RRIDAB_3670881
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP05023
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

实验方案

Product Specific Protocols
WB protocol for ATP1A1 antibody 83191-6-RRDownload protocol
IF protocol for ATP1A1 antibody 83191-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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