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ATP1A1 Recombinant antibody

ATP1A1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240160E12

Cat no : 83191-6-RR

Print datasheet

Synonyms

Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1, ATP1A 1

验证数据展示

  • WB analysis using 83191-6-RR

    WB analysis using 83191-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83191-6-RR (ATP1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 83191-6-RR

    IF Staining of HEK-293 using 83191-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP1A1 antibody (83191-6-RR, Clone: 240160E12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of A431 using 83191-6-RR

    IF Staining of A431 using 83191-6-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-6-RR, Clone: 240160E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HEK-293 using 83191-6-RR

    FC experiment of HEK-293 using 83191-6-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ATP1A1 Recombinant antibody (83191-6-RR, Clone:240160E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inunboiled A431 cells, unboiled HeLa cells, unboiled RAW 264.7 cells
Positive IF/ICC detected inA431 cells, HEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

For optimal WB detection with 83191-6-RR, we do not recommend boiling the sample after lysis.

Planning an IHC experiment? We recommend our IHCeasy ATP1A1 Ready-To-Use IHC Kit. ATP1A1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83191-6-RR targets ATP1A1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen ATP1A1 fusion protein Ag5763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Synonyms Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1, ATP1A 1
Calculated Molecular Weight 113 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC050359
Gene Symbol ATP1A1
Gene ID (NCBI) 476
ENSEMBL Gene IDENSG00000163399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP05023
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

实验方案

Product Specific Protocols
WB protocol for ATP1A1 antibody 83191-6-RRDownload protocol
IF protocol for ATP1A1 antibody 83191-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols