验证数据展示
经过测试的应用
| Positive WB detected in | unboiled A431 cells, unboiled HeLa cells, unboiled RAW 264.7 cells |
| Positive IF/ICC detected in | A431 cells, HEK-293 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
For optimal WB detection with 83191-6-RR, we do not recommend boiling the sample after lysis.
Planning an IHC experiment? We recommend our IHCeasy ATP1A1 Ready-To-Use IHC Kit. ATP1A1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83191-6-RR targets ATP1A1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IF/ICC, FC (Intra), ELISA Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | ATP1A1 fusion protein Ag5763 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | ATPase, Na+/K+ transporting, alpha 1 polypeptide |
| Synonyms | Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1, ATP1A 1 |
| Calculated Molecular Weight | 113 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC050359 |
| Gene Symbol | ATP1A1 |
| Gene ID (NCBI) | 476 |
| ENSEMBL Gene ID | ENSG00000163399 |
| RRID | AB_3670881 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P05023 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP1A1 antibody 83191-6-RR | Download protocol |
| IF protocol for ATP1A1 antibody 83191-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |